A tool for the selective sequestration of ATP and PPi to aid in-solution phosphopeptide detection assays

被引：5
作者：
Duodu, Eugenia
Kraskouskaya, Dziyana
Gomez-Biagi, Rodolfo F.
Gunning, Patrick T. [1 ]
机构：
[1] Univ Toronto, Dept Chem, 3359 Mississauga Rd North, Mississauga, ON L5L 1C6, Canada
来源：
ANALYST | 2016年 / 141卷 / 03期
基金：
加拿大自然科学与工程研究理事会;
关键词：
PHOSPHATE-CAPTURE MOLECULE; PHOSPHORYLATED PROTEINS; RECOGNITION; RECEPTORS; PEPTIDES;
D O I：
10.1039/c5an01414j
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The presence of small phospho-anions, such as PPi and ATP in protein samples often complicates the robust detection of phosphoproteins by metal-based chemosensors and receptors. We herein report the development of a bis(Zn2+-cyclen)-triethyl-benzene scaffold which can selectively sequester PPi and ATP without affecting the detection of a di-phosphorylated peptide by a ProxyPhos chemosensor.
引用
收藏
页码：820 / 822
页数：3
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