Molecular cloning and expression of β-1,4-endoxylanase gene from Chaetomium cupreum in Yeast Saccharomyces cerevisiae

被引：0
|
作者：
Zhang, Haiyan [1 ]
Li, Wenrao [1 ]
Li, Min [1 ]
机构：
[1] Henan Univ, Coll Life Sci, Kaifeng, Peoples R China
来源：
MATERIALS, TRANSPORTATION AND ENVIRONMENTAL ENGINEERING, PTS 1 AND 2 | 2013年 / 779-780卷
基金：
美国国家科学基金会;
关键词：
Chaetomium cupreum; beta-1,4-endoxylanase; Gene cloning; Saccharomyces cerevisiae H158; MICROBIAL XYLANASES; BIOTECHNOLOGY;
D O I：
10.4028/www.scientific.net/AMR.779-780.191
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The gene encoding an endo-beta-1,4-xylanase (XynCC) from chaetomium cupreum was amplified using PCR. The nucleotide sequence of a 690 bp cDNA fragment was determined. Based on the nucleotide sequence, calculated molecular mass of the enzyme was 24.7 kDa. The XynCC gene was inserted into the pYES2 vector and transferred into the cells of S. cerevisiae 11158 for heterologous expression.
引用
收藏
页码：191 / +
页数：2
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