Molecular cloning and expression of β-1,4-endoxylanase gene from Chaetomium cupreum in Yeast Saccharomyces cerevisiae

被引：0
|
作者：
Zhang, Haiyan [1 ]
Li, Wenrao [1 ]
Li, Min [1 ]
机构：
[1] Henan Univ, Coll Life Sci, Kaifeng, Peoples R China
来源：
MATERIALS, TRANSPORTATION AND ENVIRONMENTAL ENGINEERING, PTS 1 AND 2 | 2013年 / 779-780卷
基金：
美国国家科学基金会;
关键词：
Chaetomium cupreum; beta-1,4-endoxylanase; Gene cloning; Saccharomyces cerevisiae H158; MICROBIAL XYLANASES; BIOTECHNOLOGY;
D O I：
10.4028/www.scientific.net/AMR.779-780.191
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The gene encoding an endo-beta-1,4-xylanase (XynCC) from chaetomium cupreum was amplified using PCR. The nucleotide sequence of a 690 bp cDNA fragment was determined. Based on the nucleotide sequence, calculated molecular mass of the enzyme was 24.7 kDa. The XynCC gene was inserted into the pYES2 vector and transferred into the cells of S. cerevisiae 11158 for heterologous expression.
引用
收藏
页码：191 / +
页数：2
相关论文
共 50 条
  • [1] Expression of a Chitinase Gene from Chaetomium Cupreum in Saccharomyces Cerevisiae
    Zhang, Haiyan
    Ji, Hongfang
    Zhang, Lingwen
    2010 3RD INTERNATIONAL CONFERENCE ON BIOMEDICAL ENGINEERING AND INFORMATICS (BMEI 2010), VOLS 1-7, 2010, : 2045 - 2048
  • [2] MOLECULAR-CLONING OF THE ACTIN GENE FROM YEAST SACCHAROMYCES CEREVISIAE
    GALLWITZ, D
    SEIDEL, R
    NUCLEIC ACIDS RESEARCH, 1980, 8 (05) : 1043 - 1059
  • [3] MOLECULAR CLONING OF THE ACTIN GENE FROM YEAST (SACCHAROMYCES-CEREVISIAE)
    GALLWITZ, D
    HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1979, 360 (08): : 1027 - 1028
  • [4] Cloning, sequencing, and expression of a β-1,4-endoxylanase gene from Indonesian Bacillus licheniformis strain I5 in Escherichia coli
    Helianti, Is
    Nurhayati, Niknik
    Wahyuntari, Budiasih
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2008, 24 (08): : 1273 - 1279
  • [5] Cloning, sequencing, and expression of a β-1,4-endoxylanase gene from Indonesian Bacillus licheniformis strain I5 in Escherichia coli
    Is Helianti
    Niknik Nurhayati
    Budiasih Wahyuntari
    World Journal of Microbiology and Biotechnology, 2008, 24 : 1273 - 1279
  • [6] Molecular Cloning and Sequence Analysis of the Endochitinase from Chaetomium Cupreum
    Zhang, Haiyan
    Ji, Hongfang
    Zhang, Lingwen
    Wu, Xingquan
    Chen, Shihua
    2010 3RD INTERNATIONAL CONFERENCE ON BIOMEDICAL ENGINEERING AND INFORMATICS (BMEI 2010), VOLS 1-7, 2010, : 1707 - 1709
  • [7] Molecular cloning and expression of a polygalacturonase gene in Saccharomyces cerevisiae
    Hirose, N
    Kishida, M
    Kawasaki, H
    Sakai, T
    JOURNAL OF FERMENTATION AND BIOENGINEERING, 1998, 86 (03): : 332 - 334
  • [8] Molecular cloning and expression of a polygalacturonase gene in Saccharomyces cerevisiae
    Department of Applied Biochemistry, College of Agriculture, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8231, Japan
    不详
    J. Ferment. Bioeng., 3 (332-334):
  • [9] CLONING OF HUMAN LYSOZYME GENE AND EXPRESSION IN THE YEAST SACCHAROMYCES-CEREVISIAE
    CASTANON, MJ
    SPEVAK, W
    ADOLF, GR
    CHLEBOWICZSLEDZIEWSKA, E
    SLEDZIEWSKI, A
    GENE, 1988, 66 (02) : 223 - 234
  • [10] CLONING, EXPRESSION AND CHARACTERIZATION OF Pro3 GENE OF YEAST Saccharomyces cerevisiae
    姜伟东
    匡达入
    Science in China,SerB., 1988, Ser.B.1988 (07) : 828 - 837
12345