Tri-Herbal Medicine Divya Sarva-Kalp-Kwath (Livogrit) Regulates Fatty Acid-Induced Steatosis in Human HepG2 Cells through Inhibition of Intracellular Triglycerides and Extracellular Glycerol Levels

被引:4
作者
Balkrishna, Acharya [1 ,2 ,3 ]
Gohel, Vivek [1 ]
Singh, Rani [1 ]
Joshi, Monali [1 ]
Varshney, Yash [1 ]
Srivastava, Jyotish [1 ]
Bhattacharya, Kunal [1 ]
Varshney, Anurag [1 ,2 ]
机构
[1] Patanjali Res Fdn Trust, Patanjali Res Inst, Drug Discovery & Dev Div, NH-58, Haridwar 249405, Uttarakhand, India
[2] Univ Patanjali, Patanjali Yog Peeth, Dept Allied & Appl Sci, Roorkee Haridwar Rd, Haridwar 249405, Uttarakhand, India
[3] Patanjali Yog Peeth UK Trust, 40 Lambhill St,Kinning Pk, Glasgow G41 1AU, Lanark, Scotland
来源
MOLECULES | 2020年 / 25卷 / 20期
关键词
steatosis; hepatocytes; Ayurveda; Phyto-medicines; Sarva-Kalp-Kwath; Livogrit; HepG2; cells; EMBLICA L. FRUIT; LIPID-ACCUMULATION; PALMITIC ACID; LIVER-DISEASE; IN-VITRO; LIPOGENESIS; INFLAMMATION; EXPRESSION; INDUCTION; BURDEN;
D O I
10.3390/molecules25204849
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Steatosis is characterized by excessive triglycerides accumulation in liver cells. Recently, application of herbal formulations has gained importance in treating complex diseases. Therefore, this study explores the efficacy of tri-herbal medicine Divya Sarva-Kalp-Kwath (SKK; brand name, Livogrit) in treating free fatty acid (FFA)-induced steatosis in human liver (HepG2) cells and rat primary hepatocytes. Previously, we demonstrated that cytosafe SKK ameliorated CCl4-induced hepatotoxicity. In this study, we evaluated the role of SKK in reducing FFA-induced cell-death, and steatosis in HepG2 through analysis of cell viability, intracellular lipid and triglyceride accumulation, extracellular free glycerol levels, and mRNA expression changes. Plant metabolic components fingerprinting in SKK was performed via High Performance Thin Layer Chromatography (HPTLC). Treatment with SKK significantly reduced the loss of cell viability induced by 2 mM-FFA in a dose-dependent manner. SKK also reduced intracellular lipid, triglyceride accumulation, secreted AST levels, and increased extracellular free glycerol presence in the FFA-exposed cells. SKK normalized the FFA-stimulated overexpression of SREBP1c, FAS, C/EBP alpha, and CPT1A genes associated with the induction of steatosis. In addition, treatment of rat primary hepatocytes with FFA and SKK concurrently, reduced intracellular lipid accumulation. Thus, SKK showed efficacy in reducing intracellular triglyceride accumulation and increasing extracellular glycerol release, along with downregulation of related key genetic factors for FFA-associated steatosis.
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页数:18
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