Preparation of a novel molecularly imprinted polymer by a sol-gel process for on-line solid-phase extraction coupled with high performance liquid chromatography to detect trace enrofloxacin in fish and chicken samples

被引:38
作者
Junping, Wang [1 ]
Mingfei, Pan [1 ]
Guozhen, Fang [1 ]
Shuo, Wang [1 ]
机构
[1] Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China
基金
中国国家自然科学基金;
关键词
Enrofloxacin; Molecular imprinting; Sol-gel; Solid-phase extraction; High performance liquid chromatography; FLUOROQUINOLONES; QUINOLONES; HPLC; CIPROFLOXACIN; FLUORESCENCE; RECOGNITION; RESIDUES; URINE;
D O I
10.1007/s00604-009-0205-8
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A molecularly imprinted functionalized sol-gel has been prepared by using enrofloxacin (ENRO) as a template, 3-aminopropyltriethoxysilane as the functional monomer, tetraethoxysilicane as a cross-linker. This ENRO-imprinted polymer was evaluated by static, kinetic adsorption and selective experiments. The polymer displays good selectivity, and fast kinetics in terms of adsorption and desorption. The material was applied as a sorbent for on-line determination of trace ENRO by high performance liquid chromatography, with a focus on biological matrices. At a loading flow rate of 1.0 mL min(-1) for sampling 50 mL, the enrichment factor and limit of detection (LOD; at S/N = 3) are 566 and 8 ng L-1, respectively. The peak area precision (the LOD for nine replicate detections of 0.1 A mu g L-1 ENRO) is 4.4%. ENRO in fish and chicken muscle was determined at three spiked levels with recoveries ranging from 70% to 82%.
引用
收藏
页码:295 / 302
页数:8
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