In vitro and in vivo anti-retroviral activity of the substance purified from the aqueous extract of Chelidonium majus L.

被引：42

作者：

Gerencer, Marijan ^{[1
]}

Turecek, Peter L. ^{[1
]}

Kistner, Otfried ^{[1
]}

Mitterer, Artur ^{[1
]}

Savidis-Dacho, Helga ^{[1
]}

Barrett, Noel P. ^{[1
]}

机构：

[1] Baxter AG, Biomed Res Ctr, A-2304 Orth, Austria

来源：

ANTIVIRAL RESEARCH | 2006年 / 72卷 / 02期

关键词：

Chelidonium; glycosaminoglycans; HIV-1; inhibition; retroviruses; MAIDS;

D O I：

10.1016/j.antiviral.2006.03.008

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We have isolated a substance with anti-retroviral activity from the freshly prepared crude extract of Chelidonium majus L. (greater celandine) by 9-aminoacridine precipitation method and ion exchange chromatography using Dowex-50W/H+ resin followed by the gel filtration on Sephadex-75 column. Elemental and phenol/sulfuric acid method analyses as well as the mass spectrometry of the purified substance indicated that it may represent a low-sulfated poly-glycosaminoglycan moiety with molecular weight of similar to 3800 Da. The substance prevented infection of human CD4+ T-cell lines AA2 and H9 with HIV-1 at concentration of 25 mu g/mL as well as the cell-to-cell virus spread in H9 cells continuously infected with HIV-1, as determined by the measurement of reverse transcriptase activity and p24 content in cell cultures. Furthermore, we have shown in a murine AIDS model that the treatment with purified substance significantly prevented splenomegaly and the enlargement of cervical lymph nodes in C57B1/6 mice chronically infected with the pool of murine leukemia retroviruses. The mechanism(s) of anti-retroviral activity of this substance have to be elucidated. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：153 / 156

页数：4

共 19 条

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