Seed Dormancy in Arabidopsis Is Controlled by Alternative Polyadenylation of DOG1

被引：79

作者：

Cyrek, Malgorzata ^{[1
]}

Fedak, Halina ^{[1
]}

Ciesielski, Arkadiusz ^{[1
,2
]}

Guo, Yanwu ^{[1
,4
]}

Sliwa, Aleksandra ^{[1
]}

Brzezniak, Lien ^{[1
]}

Krzyczmonik, Katarzyna ^{[1
]}

Pietras, Zbigniew ^{[1
]}

Kaczanowski, Szymon ^{[1
]}

Liu, Fuquan ^{[3
]}

Swiezewski, Szymon ^{[1
]}

机构：

[1] Inst Biochem & Biophys, Dept Prot Biosynth, Pawinskiego 5a, PL-02106 Warsaw, Poland

[2] Warsaw Univ, Dept Chem, Pasteura 1, PL-02093 Warsaw, Poland

[3] Queens Univ Belfast, Sch Biol Sci, Belfast BT9 7BL, Antrim, North Ireland

[4] Friedrich Miescher Inst, CH-4002 Basel, Switzerland

来源：

PLANT PHYSIOLOGY | 2016年 / 170卷 / 02期

关键词：

ANTISENSE TRANSCRIPTS; MOLECULAR-MECHANISMS; RNA; GERMINATION; GENES; THALIANA; PROTEIN; EXPRESSION; CLEAVAGE; REVEALS;

D O I：

10.1104/pp.15.01483

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

DOG1 (Delay of Germination 1) is a key regulator of seed dormancy in Arabidopsis (Arabidopsis thaliana) and other plants. Interestingly, the C terminus of DOG1 is either absent or not conserved in many plant species. Here, we show that in Arabidopsis, DOG1 transcript is subject to alternative polyadenylation. In line with this, mutants in RNA 39 processing complex display weakened seed dormancy in parallel with defects in DOG1 proximal polyadenylation site selection, suggesting that the short DOG1 transcript is functional. This is corroborated by the finding that the proximally polyadenylated short DOG1 mRNA is translated in vivo and complements the dog1 mutant. In summary, our findings indicate that the short DOG1 protein isoform produced from the proximally polyadenylated DOG1 mRNA is a key player in the establishment of seed dormancy in Arabidopsis and characterizes a set of mutants in RNA 39 processing complex required for production of proximally polyadenylated functional DOG1 transcript.

引用

页码：947 / 955

页数：9

共 48 条

[21] A network comprising short and long noncoding RNAs and RNA helicase controls mouse retina architecture [J].

Krol, Jacek ;

Krol, Ilona ;

Alvarez, Claudia Patricia Patino ;

Fiscella, Michele ;

Hierlemann, Andreas ;

Roska, Botond ;

Filipowicz, Witold .

NATURE COMMUNICATIONS, 2015, 6

[22] Ubiquitously transcribed genes use alternative polyadenylation to achieve tissue-specific expression [J].

Lianoglou, Steve ;

Garg, Vidur ;

Yang, Julie L. ;

Leslie, Christina S. ;

Mayr, Christine .

GENES & DEVELOPMENT, 2013, 27 (21) :2380-2396

[23] An in-depth map of polyadenylation sites in cancer [J].

Lin, Yuefeng ;

Li, Zhihua ;

Ozsolak, Fatih ;

Kim, Sang Woo ;

Arango-Argoty, Gustavo ;

Liu, Teresa T. ;

Tenenbaum, Scott A. ;

Bailey, Timothy ;

Monaghan, A. Paula ;

Milos, Patrice M. ;

John, Bino .

NUCLEIC ACIDS RESEARCH, 2012, 40 (17) :8460-8471

[24] Targeted 3′ Processing of Antisense Transcripts Triggers Arabidopsis FLC Chromatin Silencing [J].

Liu, Fuquan ;

Marquardt, Sebastian ;

Lister, Clare ;

Swiezewski, Szymon ;

Dean, Caroline .

SCIENCE, 2010, 327 (5961) :94-97

[25] The absence of histone H2B monoubiquitination in the Arabidopsis hub1 (rdo4) mutant reveals a role for chromatin remodeling in seed dormancy [J].

Liu, Yongxiu ;

Koornneef, Maarten ;

Soppe, Wim J. J. .

PLANT CELL, 2007, 19 (02) :433-444

[26] Protein factors in pre-mRNA 3'-end processing [J].

Mandel, C. R. ;

Bai, Y. ;

Tong, L. .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2008, 65 (7-8) :1099-1122

[27] Altered interactions within FY/AtCPSF complexes required for Arabidopsis FCA-mediated chromatin silencing [J].

Manzano, David ;

Marquardt, Sebastian ;

Jones, Alexandra M. E. ;

Baeurle, Isabel ;

Liu, Fuquan ;

Dean, Caroline .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (21) :8772-8777

[28] Widespread Shortening of 3′UTRs by Alternative Cleavage and Polyadenylation Activates Oncogenes in Cancer Cells [J].

Mayr, Christine ;

Bartel, David P. .

CELL, 2009, 138 (04) :673-684

[29] FLOWERING LOCUS C encodes a novel MADS domain protein that acts as a repressor of flowering [J].

Michaels, SD ;

Amasino, RM .

PLANT CELL, 1999, 11 (05) :949-956

[30] The Time Required for Dormancy Release in Arabidopsis Is Determined by DELAY OF GERMINATION1 Protein Levels in Freshly Harvested Seeds [J].

Nakabayashi, Kazumi ;

Bartsch, Melanie ;

Xiang, Yong ;

Miatton, Emma ;

Pellengahr, Silke ;

Yano, Ryoichi ;

Seo, Mitsunori ;

Soppe, Wim J. J. .

PLANT CELL, 2012, 24 (07) :2826-2838

← 1 2 3 4 5 →