Virulence, antibiotic resistance and biogenic amines of bacteriocinogenic lactococci and enterococci isolated from goat milk

被引：87

作者：

Perin, Luana Martins ^{[1
]}

Miranda, Rodrigo Otavio ^{[1
]}

Todorov, Svetoslav Dimitrov ^{[2
]}

Gombossy de Melo Franco, Bernadette Dora ^{[2
]}

Nero, Luis Augusto ^{[1
]}

机构：

[1] Univ Fed Vicosa, Dept Vet, BR-36570900 Vicosa, MG, Brazil

[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo, SP, Brazil

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2014年 / 185卷

基金：

巴西圣保罗研究基金会;

关键词：

Bacteriocins; Virulence; Antibiotic resistance; Biogenic amines; Lactic acid bacteria; Goat milk; LACTIC-ACID BACTERIA; MOLECULAR CHARACTERIZATION; VANCOMYCIN RESISTANCE; DAIRY; FOOD; CHEESE; GENES; SUSCEPTIBILITY; IDENTIFICATION; DETERMINANTS;

D O I：

10.1016/j.ijfoodmicro.2014.06.001

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The present study aimed to investigate the virulence, antibiotic resistance and biogenic amine production in bacteriocinogenic lactococci and enterococci isolated from goat milk in order to evaluate their safety. Twenty-nine bacteriocinogenic lactic acid bacteria (LAB: 11 Lactococcus spp., and 18 Enterococcus spp.) isolated from raw goat milk were selected and subjected to PCR to identify gelE, cylA, hyl, asa1, esp, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc genes. The expression of virulence factors (gelatinase, hemolysis, lipase, DNAse, tyramine, histamine, putrescine) in different incubation temperatures was assessed by phenotypic methods, as well as the resistance to vancomycin, gentamicin, chloramphenicol, ampicillin and rifampicin (using Eteste (R)). The tested isolates presented distinct combinations of virulence related genes, but not necessarily the expression of such factors. The relevance of identifying virulence-related genes in bacteriocinogenic LAB was highlighted, demanding for care in their usage as starter cultures or biopreservatives due to the possibility of horizontal gene transfer to other bacteria in food systems. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：121 / 126

页数：6

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