Alteration of leukocyte populations in calves concurrently infected with bovine respiratory syncytial virus and bovine viral diarrhea virus

被引:33
作者
Brodersen, BW [1 ]
Kelling, CL [1 ]
机构
[1] Univ Nebraska, Dept Vet & Biomed Sci, Inst Agr & Nat Resources, Lincoln, NE 68583 USA
关键词
D O I
10.1089/vim.1999.12.323
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Bovine viral diarrhea virus (BVDV) infection altered leukocyte populations in calves that were reflected by depression of T, BoCD4(+), and BoCD8(+) lymphocytes in the thymus and depression of B lymphocytes in Peyer's patches (PP). The present study was based on mononuclear leukocyte preparations from eighteen 9- to 12-month-old crossbred calves that were each exposed to either bovine respiratory syncytial virus (BRSV), BVDV, or BRSV and BVDV concurrently, or served as mock-infected controls. Peripheral blood leukocytes were collected on postinfection days (PID) 0, 2, 4, 6, and 8, and cell populations from thymus, spleen, mesenteric lymph node, and PP were collected at necropsy on PID 9. The leukocytes were analyzed using flow cytometry for lymphocyte subpopulations expressing antigens specific for BoCD2, BoCD4, BoCD8, BoWC1, lambda light chain of bovine immunoglobulin, BoCD11b and major histocompatibility complex (MBC) class II. Concurrent BRSV and BVDV infections caused exaggerated alterations in leukocyte populations with a greater percentage of T-lymphocytes harvested from the PP. Alterations in the leukocyte populations in lymphatic tissues and in peripheral circulation due to BVDV infection may be an important mechanism for causation of clinically severe diseases of the respiratory and digestive tracts during concurrent BRSV and BVDV infections.
引用
收藏
页码:323 / 334
页数:12
相关论文
共 33 条
[1]  
BALDWIN CL, 1986, J IMMUNOL, V136, P4385
[2]  
BALDWIN CL, 1988, DIFFERENTIATION ANTI, P455
[3]  
BOLIN SR, 1985, AM J VET RES, V46, P573
[4]  
BOLIN SR, 1985, AM J VET RES, V46, P884
[5]  
Brodersen BW, 1998, AM J VET RES, V59, P1423
[6]   EXPERIMENTAL PRODUCTION OF FATAL MUCOSAL DISEASE IN CATTLE [J].
BROWNLIE, J ;
CLARKE, MC ;
HOWARD, CJ .
VETERINARY RECORD, 1984, 114 (22) :535-536
[7]  
BRYSON DG, 1983, AM J VET RES, V44, P1648
[8]   IDENTIFICATION OF A BOVINE SURFACE-ANTIGEN UNIQUELY EXPRESSED ON CD4-CD8- T-CELL RECEPTOR GAMMA-DELTA+ LYMPHOCYTES-T [J].
CLEVERS, H ;
MACHUGH, ND ;
BENSAID, A ;
DUNLAP, S ;
BALDWIN, CL ;
KAUSHAL, A ;
IAMS, K ;
HOWARD, CJ ;
MORRISON, WI .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1990, 20 (04) :809-817
[9]   PULMONARY HISTOPATHOLOGY INDUCED BY RESPIRATORY SYNCYTIAL VIRUS (RSV) CHALLENGE OF FORMALIN-INACTIVATED RSV-IMMUNIZED BALB/C MICE IS ABROGATED BY DEPLETION OF CD4+ T-CELLS [J].
CONNORS, M ;
KULKARNI, AB ;
FIRESTONE, CY ;
HOLMES, KL ;
MORSE, HC ;
SOTNIKOV, AV ;
MURPHY, BR .
JOURNAL OF VIROLOGY, 1992, 66 (12) :7444-7451
[10]  
CORAPI WV, 1990, AM J VET RES, V51, P1388