In Vitro Multienzymatic Reaction Systems for Biosynthesis

被引：24

作者：

Ardao, Ines ^{[1
]}

Hwang, Ee Taek ^{[1
]}

Zeng, An-Ping ^{[1
]}

机构：

[1] Tech Univ Hamburg, Inst Bioproc & Biosyst Engn, D-21073 Hamburg, Germany

来源：

FUNDAMENTALS AND APPLICATION OF NEW BIOPRODUCTION SYSTEMS | 2013年 / 137卷

关键词：

Co-immobilization; Enzyme microreactors; In vitro multienzymatic systems; Multienzyme process design; Nano/micro-sized materials; Synthetic metabolic pathways; ONE-POT; COFACTOR REGENERATION; POLYMERSOME NANOREACTORS; ENZYMATIC MICROREACTOR; MICROFLUIDIC CHANNELS; EFFICIENT CONVERSION; SYNTHETIC BIOLOGY; CASCADE REACTIONS; DEHYDROGENASES; CARBON-DIOXIDE;

D O I：

10.1007/10_2013_232

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In vitro multienzymatic bioreaction systems are attracting increasing attention for the development of bioproduction systems. The de-coupling of the biocatalytic pathway from the cellular machinery aimed at growth and survival allows achievement of high product yields and thus reduces byproduct or waste generation. Additionally, the use of several enzymes allows the realization of much more complex synthetic schemes, thus expanding the chemical diversity of synthetic compounds with new chemical properties or bioactivities. This chapter provides a survey of in vitro multienzymatic bioreaction systems used for biosynthesis, discusses process design aspects for the technical realization of multienzymatic bioreactions, highlights advantages and recent developments in using multienzyme microreactors, and finally reviews examples and strategies of the co-immobilization of multienzymes in nano/microsized materials.

引用

页码：153 / 184

页数：32

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