Inconsistencies and ambiguities in calculating enzyme activity: The case of laccase

被引：51

作者：

Baltierra-Trejo, Eduardo ^{[2
,3
]}

Marquez-Benavides, Liliana ^{[2
,4
]}

Sanchez-Yanez, Juan Manuel ^{[1
,3
]}

机构：

[1] Univ Michoacana, Inst Chem Biol Res, Morelia 58040, Michoacan, Mexico

[2] Univ Michoacana, IIAF, Solid Waste Res Grp, Morelia 58302, Michoacan, Mexico

[3] Univ Michoacana, IIQB, Environm Microbiol Res Grp, Morelia 58040, Michoacan, Mexico

[4] Mexican Soc Sci & Technol Appl Solid Waste, Delegacion Cuauhtemoc 06700, DF, Mexico

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 119卷

关键词：

Enzyme activity; Ascomycete; Basidiomycete; Ligninolytic; Laccase; LIGNIN; ASCOMYCETE; DEGRADATION; PURIFICATION; PEROXIDASE;

D O I：

10.1016/j.mimet.2015.10.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Laccase is a key enzyme in the degradation of lignin by fungi. Reports indicate that the activity of this enzyme ranges from 3.5 to 484,000 U L-1. Our aim was to analyze how laccase activity is calculated in the literature, and to determine statistically whether variations in activity are due to biological properties or to inconsistencies in calculation. We found a general lack of consensus on the definition of enzyme activity, and enzymes are sometimes characterized in terms of reaction rate and specific activity. Moreover, enzyme activity is calculated using at least seven different equations. Therefore, it is critical to standardize the calculation of laccase activity in order to compare results directly. (C) 2015 Published by Elsevier B.V.

引用

页码：126 / 131

页数：6

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