MicroRNA-543 suppresses endometrial cancer oncogenicity via targeting FAK and TWIST1 expression

被引：77

作者：

Li Bing ^{[1
]}

Chen Hong ^{[2
]}

Shang Li-Xin ^{[1
]}

Gao Wei ^{[3
]}

机构：

[1] Mil Gen Hosp Beijing PLA, Dept Gynecol & Obstet, Beijing 100700, Peoples R China

[2] Mil Gen Hosp Beijing PLA, Dept Anesthesiol, Beijing 100700, Peoples R China

[3] Beijing Matern Hosp, Dept Gynecol, Beijing 100026, Peoples R China

来源：

ARCHIVES OF GYNECOLOGY AND OBSTETRICS | 2014年 / 290卷 / 03期

关键词：

MicroRNA-543; Endometrial cancer; FAK; TWIST1; FOCAL-ADHESION KINASE; BREAST-CANCER; MESENCHYMAL TRANSITION; METASTASIS; OVEREXPRESSION; INVASION; CLUSTER;

D O I：

10.1007/s00404-014-3219-3

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Focal adhesion kinase (FAK) is a critical mediator of extracellular matrix signaling, cell survival, proliferation and motility. Twist homolog 1 (TWIST1) is a transcription factor and serves as a powerful oncogene. Increased FAK and TWIST1 expression are observed in a variety of solid human tumors and correlate with metastasis and poor survival. Here, we identify miR-543 as a direct regulator of FAK and TWIST1 expression in endometrial cancer. Forced expression of miR-543 in endometrial cancer cell lines decreases both endogenous FAK and TWIST1 mRNA and protein levels. Forced expression of miR-543 in aggressive endometrial cancer cell lines also impairs tumor cell monolayer proliferation, anchorage-independent growth, migration and invasion. Endogenous miR-543 expression is decreased in malignant versus normal endometrium tissue and the levels of miR-543 inversely correlate with mRNA levels of FAK and TWIST1. miR-543 expression is decreased in endometrial cancer and serves as a tumor suppressor by targeting FAK and TWIST1 expression.

引用

页码：533 / 541

页数：9

共 32 条

[1] Endometrial cancer [J].

Amant, F ;

Moerman, P ;

Neven, P ;

Timmerman, D ;

Van Limbergen, E ;

Vergote, I .

LANCET, 2005, 366 (9484) :491-505

[2] MicroRNAs: Target Recognition and Regulatory Functions [J].

Bartel, David P. .

CELL, 2009, 136 (02) :215-233

[3]

Cance WG, 2000, CLIN CANCER RES, V6, P2417

[4] A Twist in fate: evolutionary comparison of Twist structure and function [J].

Castanon, I ;

Baylies, MK .

GENE, 2002, 287 (1-2) :11-22

[5] Gain of miR-151 on chromosome 8q24.3 facilitates tumour cell migration and spreading through downregulating RhoGDIA [J].

Ding, Jie ;

Huang, Shenglin ;

Wu, Shunquan ;

Zhao, Yingjun ;

Liang, Linhui ;

Yan, Mingxia ;

Ge, Chao ;

Yao, Jian ;

Chen, Taoyang ;

Wan, Dafang ;

Wang, Hongyang ;

Gu, Jianren ;

Yao, Ming ;

Li, Jinjun ;

Tu, Hong ;

He, Xianghuo .

NATURE CELL BIOLOGY, 2010, 12 (04) :390-U208

[6] The evolution of the DLK1-DIO3 imprinted domain in mammals [J].

Edwards, Carol A. ;

Mungall, Andrew J. ;

Matthews, Lucy ;

Ryder, Edward ;

Gray, Dionne J. ;

Pask, Andrew J. ;

Shaw, Geoffrey ;

Graves, Jennifer A. M. ;

Rogers, Jane ;

Dunham, Ian ;

Renfree, Marilyn B. ;

Ferguson-Smith, Anne C. .

PLOS BIOLOGY, 2008, 6 (06) :1292-1305

[7] Expression of Focal Adhesion Kinase in Patients With Endometrial Cancer A Clinicopothologic Study [J].

Gabriel, Boris ;

Hasenburg, Annette ;

Waizenegger, Miriam ;

Orlowska-Volk, Marzenna ;

Stickeler, Elmar ;

zur Hausen, Axel .

INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2009, 19 (07) :1221-1225

[8] MicroRNA targeting specificity in mammals: Determinants beyond seed pairing [J].

Grimson, Andrew ;

Farh, Kyle Kai-How ;

Johnston, Wendy K. ;

Garrett-Engele, Philip ;

Lim, Lee P. ;

Bartel, David P. .

MOLECULAR CELL, 2007, 27 (01) :91-105

[9] Cancer metastasis:: Building a framework [J].

Gupta, Gaorav P. ;

Massague, Joan .

CELL, 2006, 127 (04) :679-695

[10] MicroRNAs in the Imprinted DLK1-DIO3 Region Repress the Epithelial-to-Mesenchymal Transition by Targeting the TWIST1 Protein Signaling Network [J].

Haga, Christopher L. ;

Phinney, Donald G. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (51) :42695-42707

← 1 2 3 4 →