Nanoscale three-dimensional single particle tracking by light-sheet-based double-helix point spread function microscopy

被引：20

作者：

Yu, Bin ^{[1
,2
]}

Yu, Jie ^{[1
,2
]}

Li, Weihai ^{[1
,2
]}

Cao, Bo ^{[1
,2
]}

Li, Heng ^{[1
,2
]}

Chen, Danni ^{[1
,2
]}

Niu, Hanben ^{[1
,2
]}

机构：

[1] Shenzhen Univ, Coll Optoelect Engn, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China

[2] Shenzhen Univ, Coll Optoelect Engn, Shenzhen Key Lab Micronano Measuring & Imaging Bi, Shenzhen 518060, Peoples R China

来源：

APPLIED OPTICS | 2016年 / 55卷 / 03期

基金：

中国国家自然科学基金;

关键词：

FLUORESCENCE MICROSCOPY; LOCALIZATION ACCURACY; DIMENSIONS;

D O I：

10.1364/AO.55.000449

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The double-helix point spread function (DH-PSF) microscopy has become an essential tool for nanoscale three-dimensional (3D) localization and tracking of single molecules in living cells. However, its localization precision is limited by fluorescent contrast in thick samples because the signal-to-noise ratio of the system is low due to the inherent low transfer function efficiency and background fluorescence. Here we combine DH-PSF microscopy with light-sheet illumination to eliminate out-of-focus background fluorescence for high-precision 3D single particle tracking. To demonstrate the capability of the method, we obtain the single fluorescent bead image with light-sheet illumination, with three-dimensional localization accuracy better than that of epi-illumination. We also show that the single fluorescent beads in agarose solution can be tracked, which demonstrates the possibility of our method for the study of dynamic processes in complex biological specimens. (C) 2016 Optical Society of America

引用

页码：449 / 453

页数：5