Theory of time-resolved single-molecule fluorescence spectroscopy

被引：39
|
作者：
Molski, A
Hofkens, J
Gensch, T
Boens, N
De Schryver, F
机构：
[1] Adam Mickiewicz Univ, Dept Phys Chem, PL-60780 Poznan, Poland
[2] Katholieke Univ Leuven, Dept Chem, B-3001 Heverlee, Belgium
来源：
CHEMICAL PHYSICS LETTERS | 2000年 / 318卷 / 4-5期
关键词：
D O I：
10.1016/S0009-2614(00)00040-3
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The statistics of time-resolved photon detection in single-molecule continuous-excitation fluorescence spectroscopy is examined for three- and two-state models of a dye molecule. A unified description is provided in terms of stationary stochastic point processes. The pair distribution function and the distribution of interdetection times are related to the parameters of the models, and explicit expressions are given for the two-state model. The effect of the detected background is taken into account. Based on an analysis of tripler blinking, we argue that time-resolved photon detection offers an alternative to fluorescence intensity spectroscopy in unravelling the underlying single-molecule processes. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：325 / 332
页数：8
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