Low expression of mitofusin 1 is associated with mitochondrial dysfunction and apoptosis in porcine somatic cell nuclear transfer embryos

被引：5

作者：

Park, Mi-Ryung ^{[1
]}

Hwang, In-Sul ^{[1
]}

Kwak, Tae-Uk ^{[1
]}

Lim, Ji-Hyun ^{[1
]}

Hwang, Seongsoo ^{[1
]}

Cho, Seong-Keun ^{[2
]}

机构：

[1] Natl Inst Anim Sci, Rural Dev Adm, Anim Biotechnol Div, 1500 Kongjwipatjwi Ro, Iseo Myeon 55365, Jeollabuk Do, South Korea

[2] Pusan Univ, Coll Nat Sci, Dept Anim Sci Life & Ind Convergence, Res Inst RICRI, Miryang 50463, Gyeongnam, South Korea

来源：

ANIMAL SCIENCE JOURNAL | 2020年 / 91卷 / 01期

关键词：

mitochondria; mitofusin; 1; porcine; SCNT; FISSION; OOCYTE; MOUSE; DYNAMICS; PROTEIN; FUSION; METABOLISM; INHIBITOR; DRP1;

D O I：

10.1111/asj.13430

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Mitochondria are necessary for the transition from oocyte to embryo and for early embryonic development. Mitofusin 1 is the main mediator of mitochondrial fusion and homeostasis. We investigated Mitofusin 1 expression levels in porcine somatic cell nuclear transfer (SCNT) embryos. The rate of blastocyst formation in SCNT embryos was reduced significantly compared with that of parthenogenetic activation embryos. SCNT embryos showed significantly decreased Mitofusin 1 expression and mitochondrial membrane potential, while exhibiting increased reactive oxygen species and apoptosis. Mitochondrial functional changes were observed in the SCNT embryos and may be correlated with low levels of Mitofusin 1 to negatively affect development.

引用

页数：10

共 50 条

[41] Microtubule distribution in somatic cell nuclear transfer bovine embryos following control of nuclear remodeling type [J].

Kwon, Dae-Jin ;

Lee, Yu-Mi ;

Hwang, In-Sun ;

Park, Choon-Keun ;

Yang, Boo-Keun ;

Cheong, Hee-Tae .

JOURNAL OF VETERINARY SCIENCE, 2010, 11 (02) :93-101

[42] Telomere Elongation Facilitated by Trichostatin A in Cloned Embryos and Pigs by Somatic Cell Nuclear Transfer [J].

Qingran Kong ;

Guangzhen Ji ;

Bingteng Xie ;

Jingyu Li ;

Jian Mao ;

Juan Wang ;

Shichao Liu ;

Lin Liu ;

Zhonghua Liu .

Stem Cell Reviews and Reports, 2014, 10 :399-407

[43] Reprogrammed Transcriptome in Rhesus-Bovine Interspecies Somatic Cell Nuclear Transfer Embryos [J].

Wang, Kai ;

Otu, Hasan H. ;

Chen, Ying ;

Lee, Young ;

Latham, Keith ;

Cibelli, Jose B. .

PLOS ONE, 2011, 6 (07)

[44] Human Embryos Derived by Somatic Cell Nuclear Transfer Using an Alternative Enucleation Approach [J].

Li, Jianyuan ;

Liu, Xuexia ;

Wang, Haiyan ;

Zhang, Shouxin ;

Liu, Fujun ;

Wang, Xuebo ;

Wang, Yanwei .

CLONING AND STEM CELLS, 2009, 11 (01) :39-50

[45] Lack of effects of ooplasm transfer on early development of interspecies somatic cell nuclear transfer bison embryos [J].

Gonzalez-Grajales, L. Antonio ;

Favetta, Laura A. ;

King, W. Allan ;

Mastromonaco, Gabriela F. .

BMC DEVELOPMENTAL BIOLOGY, 2016, 16

[46] Effect of histone acetylation modification with MGCD0103, a histone deacetylase inhibitor, on nuclear reprogramming and the developmental competence of porcine somatic cell nuclear transfer embryos [J].

Jin, Long ;

Zhu, Hai-Ying ;

Guo, Qing ;

Li, Xiao-Chen ;

Zhang, Yu-Chen ;

Cui, Cheng-Du ;

Li, Wen-Xue ;

Cui, Zheng-Yun ;

Yin, Xi-Jun ;

Kang, Jin-Dan .

THERIOGENOLOGY, 2017, 87 :298-305

[47] Effects of scriptaid on the histone acetylation of buffalo oocytes and their ability to support the development of somatic cell nuclear transfer embryos [J].

Sun, Hongliang ;

Lu, Fenghua ;

Liu, Xiaohua ;

Tian, Mingming ;

Ruan, Ziyun ;

Zhu, Peng ;

Ruan, Qiuyan ;

Jiang, Jianrong ;

Shi, Deshun .

THERIOGENOLOGY, 2015, 83 (07) :1219-1225

[48] Expression patterns of mitochondrial OXPHOS components, mitofusin 1 and dynamin-related protein 1 are associated with human embryo fragmentation [J].

Otasevic, Vesna ;

Surlan, Lela ;

Vucetic, Milica ;

Tulic, Ivan ;

Buzadzic, Biljana ;

Stancic, Ana ;

Jankovic, Aleksandra ;

Velickovic, Ksenija ;

Golic, Igor ;

Markelic, Milica ;

Korac, Aleksandra ;

Korac, Bato .

REPRODUCTION FERTILITY AND DEVELOPMENT, 2016, 28 (03) :319-327

[49] Visualization of endogenous nuclear F-actin in mouse embryos reveals abnormal actin assembly after somatic cell nuclear transfer [J].

Shindo, Taiki ;

Ihashi, Shunya ;

Sakamoto, Yuko ;

Okuno, Tomomi ;

Tomikawa, Junko ;

Miyamoto, Kei .

JOURNAL OF BIOCHEMISTRY, 2021, 169 (03) :303-311

[50] Low expression of Mfn2 is associated with mitochondrial damage and apoptosis in the placental villi of early unexplained miscarriage [J].

Pang, W. ;

Zhang, Y. ;

Zhao, N. ;

Darwiche, S. S. ;

Fu, X. ;

Xiang, W. .

PLACENTA, 2013, 34 (07) :613-618

← 1 2 3 4 5 →