Low expression of mitofusin 1 is associated with mitochondrial dysfunction and apoptosis in porcine somatic cell nuclear transfer embryos

被引：5
作者：
Park, Mi-Ryung [1 ]
Hwang, In-Sul [1 ]
Kwak, Tae-Uk [1 ]
Lim, Ji-Hyun [1 ]
Hwang, Seongsoo [1 ]
Cho, Seong-Keun [2 ]
机构：
[1] Natl Inst Anim Sci, Rural Dev Adm, Anim Biotechnol Div, 1500 Kongjwipatjwi Ro, Iseo Myeon 55365, Jeollabuk Do, South Korea
[2] Pusan Univ, Coll Nat Sci, Dept Anim Sci Life & Ind Convergence, Res Inst RICRI, Miryang 50463, Gyeongnam, South Korea
来源：
ANIMAL SCIENCE JOURNAL | 2020年 / 91卷 / 01期
关键词：
mitochondria; mitofusin; 1; porcine; SCNT; FISSION; OOCYTE; MOUSE; DYNAMICS; PROTEIN; FUSION; METABOLISM; INHIBITOR; DRP1;
D O I：
10.1111/asj.13430
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Mitochondria are necessary for the transition from oocyte to embryo and for early embryonic development. Mitofusin 1 is the main mediator of mitochondrial fusion and homeostasis. We investigated Mitofusin 1 expression levels in porcine somatic cell nuclear transfer (SCNT) embryos. The rate of blastocyst formation in SCNT embryos was reduced significantly compared with that of parthenogenetic activation embryos. SCNT embryos showed significantly decreased Mitofusin 1 expression and mitochondrial membrane potential, while exhibiting increased reactive oxygen species and apoptosis. Mitochondrial functional changes were observed in the SCNT embryos and may be correlated with low levels of Mitofusin 1 to negatively affect development.
引用
收藏
页数：10
相关论文
共 50 条
  • [31] Ascorbic acid increases demethylation in somatic cell nuclear transfer embryos of the pig (Sus scrofa)
    Zhao, Minghui
    Hur, Tai-Young
    No, Jingu
    Nam, Yoonseok
    Kim, Hyeunkyu
    Im, Gi-Sun
    Lee, Seunghoon
    [J]. ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 2017, 30 (07): : 944 - 949
  • [32] Scriptaid Upregulates Expression of Development-Related Genes, Inhibits Apoptosis, and Improves the Development of Somatic Cell Nuclear Transfer Mini-Pig Embryos
    Zhang, Li
    Huang, Yuemeng
    Wu, Yanjun
    Si, Jinglei
    Huang, Yanna
    Jiang, Qinyang
    Lan, Ganqiu
    Guo, Yafen
    Jiang, Hesheng
    [J]. CELLULAR REPROGRAMMING, 2017, 19 (01) : 19 - 26
  • [33] Melatonin enhances the developmental competence of porcine somatic cell nuclear transfer embryos by preventing DNA damage induced by oxidative stress
    Liang, Shuang
    Jin, Yong-Xun
    Yuan, Bao
    Zhang, Jia-Bao
    Kim, Nam-Hyung
    [J]. SCIENTIFIC REPORTS, 2017, 7
  • [34] A-type lamin dynamics in bovine somatic cell nuclear transfer embryos
    Kelly, Richard D. W.
    Alberio, Ramiro
    Campbell, Keith H. S.
    [J]. REPRODUCTION FERTILITY AND DEVELOPMENT, 2010, 22 (06) : 956 - 965
  • [35] Cardiomyocyte deletion of mitofusin-1 leads to mitochondrial fragmentation and improves tolerance to ROS-induced mitochondrial dysfunction and cell death
    Papanicolaou, Kyriakos N.
    Ngoh, Gladys A.
    Dabkowski, Erinne R.
    O'Connell, Kelly A.
    Ribeiro, Rogerio F., Jr.
    Stanley, William C.
    Walsh, Kenneth
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2012, 302 (01): : H167 - H179
  • [36] Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos
    Li, Yanhe
    Zheng, Caihong
    Liu, Yingdong
    He, Jincan
    Zhang, Qiang
    Zhang, Yalin
    Kou, Xiaochen
    Zhao, Yanhong
    Liu, Kuisheng
    Bai, Dandan
    Jia, Yanping
    Han, Xiaoxiao
    Sheng, Yifan
    Yin, Jiqing
    Wang, Hong
    Gao, Shuai
    Liu, Wenqiang
    Gao, Shaorong
    [J]. NATIONAL SCIENCE REVIEW, 2023, 10 (09)
  • [37] Mitochondrial metabolism assessment of lycaon-dog fetuses in interspecies somatic cell nuclear transfer
    Son, Young-Bum
    Jeong, Yeon Ik
    Hwang, Kyu Chan
    Jeong, Yeon Woo
    Suk, Woo
    [J]. THERIOGENOLOGY, 2021, 165 : 18 - 27
  • [38] Sequential treatment with resveratrol-trolox improves development of porcine embryos derived from parthenogenetic activation and somatic cell nuclear transfer
    Lee, Sanghoon
    Park, Eun Jung
    Moon, Joon Ho
    Kim, Su Jin
    Song, Kilyoung
    Lee, Byeong Chun
    [J]. THERIOGENOLOGY, 2015, 84 (01) : 145 - 154
  • [39] Day-3 Medium Changes can Affect Developmental Potential of Porcine Somatic Cell Nuclear Transfer and Parthenogenesis Embryos In Vitro
    Biswas, Dibyendu
    Hyun, Sang Hwan
    [J]. PAKISTAN VETERINARY JOURNAL, 2011, 31 (01) : 27 - 30
  • [40] DRP1 deficiency induces mitochondrial dysfunction and oxidative stress-mediated apoptosis during porcine oocyte maturation
    Zhang, Haolin
    Pan, Zhennan
    Ju, Jiaqian
    Xing, Chunhua
    Li, Xiaohan
    Shan, Mengmeng
    Sun, Shaochen
    [J]. JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY, 2020, 11 (01)
12345