Cytokine production in human periodontal ligament cells stimulated with Porphyromonas gingivalis

Background and Objective: Although some functions and characterizations of human periodontal ligament (hPDL) cells have been reported, the role of hPDL cells in periodontal disease is poorly understood. We have previously reported that hPDL cells produce many kinds of inflammatory cytokines by stimulation with Prevotella intermedia. In this study, we examined the production of cytokines in hPDL cells stimulated with Porphyromonas gingivalis as compared with P. intermedia. Material and Methods: hPDL cells cultured in Dulbecco's modified Eagles's medium (DMEM) containing 10% fetal bovine serum (FBS) and antibiotics. After three to four passages, hPDL cells were stimulated with P. intermedia (ATCC25601) or P. gingivalis (ATCC33277) for 24 h. Total RNA was extracted by ISOGEN and the expression of cytokine mRNA was determined using reverse transcription-polymerase chain reaction. Cytokines in the culture supernatants were assessed by enzyme-linked immunosorbent assay. Results: The expression of interleukin-1 beta, interleukin-6, interleukin-8, tumor necrosis factor-alpha (TNF-alpha), receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) mRNA was detected in hPDL cells after stimulation with P. gingivalis as well as P. intermedia. There were no significant differences in the kind of cytokines expressed in hPDL cells between P. gingivalis and P. intermedia. However, P. gingivalis induced a significantly higher production of cytokines in hPDL cells than P. intermedia (p < 0.05). Conclusion: This study demonstrated that hPDL cells produce many kinds of cytokines as a result of bacterial stimulation, including stimulation with P. gingivalis and P. intermedia. These results suggest that hPDL cells may play a role in cytokine production in periodontal disease.