Identification of Listeria species by a semi-nested polymerase chain reaction

被引：12

作者：

Manzano, M

Cocolin, L

Ferroni, P

Gasparini, V

Narduzzi, D

Cantoni, C

Comi, G

机构：

[1] UNIV UDINE,FAC AGRARIA,DIPARTIMENTO SCI ALIMENTI,I-33100 UDINE,ITALY

[2] UNIV UDINE,DIPARTIMENTO PATOL & MED SPERIMENTALE,I-33100 UDINE,ITALY

[3] UNIV UDINE,CLIN FAC MED & CHIRURG,I-33100 UDINE,ITALY

[4] PRESIDIO MULTIZONALE PREVENZ,I-33100 UDINE,ITALY

[5] UNIV MILAN,FAC MED VET,IST ISPE ALIMENTI ORIGINE ANIM,I-20121 MILAN,ITALY

来源：

RESEARCH IN MICROBIOLOGY | 1996年 / 147卷 / 08期

关键词：

PCR; Listeria; iap gene; identification;

D O I：

10.1016/0923-2508(96)84020-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L. innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welshimeri.

引用

页码：637 / 640

页数：4

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