Immunoarchitectural characterization of a human skin model reconstructed in vitro

被引:6
作者
Martinhao Souto, Luis Ricardo [1 ]
Vassallo, Jose [1 ]
Rehder, Jussara [1 ]
Pinto, Glauce Aparecida [1 ]
Puzzi, Maria Beatriz [1 ]
机构
[1] Univ Estadual Campinas, UNICAMP, Ctr Invest Pediat, Skin Cell Culture Lab, Sao Paulo, Brazil
来源
SAO PAULO MEDICAL JOURNAL | 2009年 / 127卷 / 01期
关键词
Burns; Cytokeratin; Immunohistochemistry; Skin transplantation; Tissue engineering; EPIDERMAL KERATINS; ARTIFICIAL SKIN; DERMAL TEMPLATE; HAIR-FOLLICLES; EXPRESSION; EQUIVALENTS; FIBROBLASTS; CELLS; MELANOCYTES; MEMBRANE;
D O I
10.1590/S1516-31802009000100007
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
CONTEXT AND OBJECTIVE: Over the last few years, different models for human skin equivalent reconstructed in vitro (HSERIV) have been reported for clinical usage and applications in research for the pharmaceutical industry. Before release for routine use as human skin replacements, HSERIV models need to be tested regarding their similarity with in vivo skin, using morphological (architectural) and immunohistochemical (functional) analyses. A model for HSERIV has been developed in our hospital, and our aim here was to further characterize its immunoarchitectural features by comparing them with human skin, before it can be tested for clinical use, e.g. for severe burns or wounds, whenever ancillary methods are not indicated. DESIGN AND SETTING: Experimental laboratory study, in the Skin Cell Culture Laboratory, School of Medical Sciences, Universidade Estadual de Campinas. METHODS: Histological sections were stained with hematoxylin-eosin, Masson's trichrome for collagen fibers, periodic acid-Schiff reagent for basement membrane and glycogen, Weigert-Van Gieson for elastic fibers and Fontana-Masson for melanocytes. Immunohistochemistry was used to localize cytokeratins (broad spectrum of molecular weight, AE1/AE3), high molecular weight Cytokeratins (34 beta E12), low molecular weight cytokeratins (35 beta H11), cytokeratins 7 and 20, vimentin, S-100 protein (for melanocytic and dendritic cells), CD68 (KP1, histiocytes) and CD34 (QBend, endothelium). RESULTS: Histology revealed satisfactory similarity between HSERIV and in vivo skin. Immunohistochemical analysis on HSERIV demonstrated that the marker pattern was similar to what is generally present in human skin in vivo. CONCLUSION: HSERIV is morphologically and functionally compatible with human skin observed in vivo.
引用
收藏
页码:28 / 33
页数:6
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