Tracking protein turnover and degradation by microscopy: photo-switchable versus time-encoded fluorescent proteins

被引：9
作者：
Knop, Michael [1 ]
Edgar, Bruce A. [1 ]
机构：
[1] Heidelberg Univ, Zentrum Mol Biol, Deutsch Krebsforschungszentrum, DKFZ ZMBH Allianz, D-69120 Heidelberg, Germany
来源：
OPEN BIOLOGY | 2014年 / 4卷 / 04期
关键词：
protein dynamics; fluorescent timer proteins; live cell microscopy; photo-switchable fluorescent proteins; COLOR; CHASE;
D O I：
10.1098/rsob.140002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Expanded fluorescent protein techniques employing photo-switchable and fluorescent timer proteins have become important tools in biological research. These tools allow researchers to address a major challenge in cell and developmental biology, namely obtaining kinetic information about the processes that determine the distribution and abundance of proteins in cells and tissues. This knowledge is often essential for the comprehensive understanding of a biological process, and/or required to determine the precise point of interference following an experimental perturbation.
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页数：4
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