Whole Genome Sequencing of the Giant Grouper (Epinephelus lanceolatus) and High-Throughput Screening of Putative Antimicrobial Peptide Genes

被引:29
作者
Wang, Dengdong [1 ,2 ]
Chen, Xiyang [3 ,4 ]
Zhang, Xinhui [4 ]
Li, Jia [4 ]
Yi, Yunhai [3 ,4 ]
Bian, Chao [4 ]
Shi, Qiong [3 ,4 ,5 ]
Lin, Haoran [1 ,2 ]
Li, Shuisheng [1 ,2 ]
Zhang, Yong [1 ,2 ]
You, Xinxin [3 ,4 ]
机构
[1] Sun Yat Sen Univ, State Key Lab Biocontrol, Guangdong Prov Key Lab Aquat Econ Anim & Guangdon, Res Ctr Hlth Breeding Important Econ Fish,Sch Lif, Guangzhou 510275, Guangdong, Peoples R China
[2] Guangdong Ocean Univ, Zhanjiang Bay Lab, Guangdong Res Ctr Reprod Control & Breeding Techn, Fisheries Coll, Zhanjiang 524088, Peoples R China
[3] Univ Chinese Acad Sci, BGI Educ Ctr, Shenzhen 518083, Peoples R China
[4] BGI Acad Marine Sci, Shenzhen Key Lab Marine Genom, Guangdong Prov Key Lab Mol Breeding Marine Econ A, BGI Marine,BGI, Shenzhen 518083, Peoples R China
[5] Shenzhen Univ, Lab Aquat Genom, Coll Life Sci & Oceanog, Shenzhen 518060, Peoples R China
基金
中国国家自然科学基金;
关键词
giant grouper; Epinephelus lanceolatus; genome sequencing; antimicrobial peptide; growth; ORANGE-SPOTTED GROUPER; PROVIDES INSIGHTS; EXPRESSION; PREDICTION; IDENTIFICATION; EPINECIDIN-1; SIGNATURES; EVOLUTION; SELECTION; ALIGNMENT;
D O I
10.3390/md17090503
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Giant groupers, the largest grouper type in the world, are of economic importance in marine aquaculture for their rapid growth. At the same time, bacterial and viral diseases have become the main threats to the grouper industry. Here, we report a high-quality genome of a giant grouper sequenced by an Illumina HiSeq X-Ten and PacBio Bioscience Sequel platform. A total of 254 putative antimicrobial peptide (AMP) genes were identified, which can be divided into 34 classes according to the annotation of the Antimicrobial Peptides Database (APD3). Their locations in pseudochromosomes were also determined. Thrombin-, lectin-, and scolopendin-derived putative AMPs were the three largest parts. In addition, expressions of putative AMPs were measured by our transcriptome data. Two putative AMP genes (gapdh1 and gapdh2) were involved in glycolysis, which had extremely high expression levels in giant grouper muscle. As it has been reported that AMPs inhibit the growth of a broad spectrum of microbes and participate in regulating innate and adaptive immune responses, genome sequencing of this study provides a comprehensive cataloging of putative AMPs of groupers, supporting antimicrobial research and aquaculture therapy. These genomic resources will be beneficial to further molecular breeding of this economically important fish.
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页数:16
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