Changes in lead availability affect bacterial community structure but not basal respiration in a microcosm study with forest soils

被引:29
作者
Lazzaro, A.
Schulin, R.
Widmer, F.
Frey, B.
机构
[1] Swiss Fed Inst Forest Snow & Landscape Res, WSL, CH-8903 Birmensdorf, Switzerland
[2] ETH, Swiss Fed Inst Technol, Dept Environm Sci DUWIS, ITE, CH-8092 Zurich, Switzerland
[3] Swiss Fed Res Stn Agroecol & Agr, Agroscope FAL Rockenholz, CH-8046 Zurich, Switzerland
关键词
lead; bioavailability; T-RFLP;
D O I
10.1016/j.scitotenv.2006.08.033
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
This study investigates the effects of Pb during time on the bacterial communities of forest soils using water-extractable Pb concentrations in the soil solution as predictors of Pb bioavailability. In a microcosm experiment we applied increasing concentrations of Pb(NO3)(2) solutions (0.5, 2, 8, 32 mM) to 5 forest soils of pH < 5 and to a calcareous soil of pH > 6.5. Sampling of the microcosms was performed after 3, 30 and 90 days of incubation. Community analysis included basal respiration rates and changes in the structure of the bacterial communities through T-RFLP fingerprinting. We also investigated functional stability in terms of resistance, expressed as the effects on basal respiration after 3 days of incubation, and of resilience, expressed as the recovery of bacterial community structure and of respiration rates after 90 days of incubation. Water-extractable Pb increased with time in most of the soils, in parallel with an increase of water-extractable dissolved organic carbon (DOC). The increased concentrations slightly affected bacterial community structure, although OTU (operational taxonomic unit) richness was not significantly reduced with Pb concentrations in any of the soils. The highest Pb treatment (32 mM) caused significant effects on basal respiration in some of the acidic soils, but no clear trend was observed in relation to increased Pb bioavailability with time. Resistance to Pb additions was evident in five of the six soils, but only two showed resilience after 90 days. This is the first study showing the effects of time on Pb bioavailability in soils and on the resulting reactions of the soil microbial communities. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:110 / 124
页数:15
相关论文
共 72 条
[1]   Effects of lead and cadmium nitrate on biomass and substrate utilization pattern of soil microbial communities [J].
Akmal, M ;
Xu, JM ;
Li, ZJ ;
Wang, HZ ;
Yao, HY .
CHEMOSPHERE, 2005, 60 (04) :508-514
[3]   Critical examination of trace element enrichments and depletions in soils: As, Cr, Cu, Ni, Pb, and Zn in Swiss forest soils [J].
Blaser, P ;
Zimmermann, S ;
Luster, J ;
Shotyk, W .
SCIENCE OF THE TOTAL ENVIRONMENT, 2000, 249 (1-3) :257-280
[4]  
BLASER P, 2005, WALDBODEN SCHWEIZ, V2
[5]   Effects on mor layer respiration by small experimental additions of mercury and lead [J].
Bringmark, L ;
Bringmark, E ;
Samuelsson, B .
SCIENCE OF THE TOTAL ENVIRONMENT, 1998, 213 (1-3) :115-119
[6]   Metal ions speciation in a soil and its solution:: experimental data and model results [J].
Cancès, B ;
Ponthieu, M ;
Castrec-Rouelle, M ;
Aubry, E ;
Benedetti, MF .
GEODERMA, 2003, 113 (3-4) :341-355
[7]   Phylogenetic specificity and reproducibility and new method for analysis of terminal restriction fragment profiles of 16S rRNA genes from bacterial communities [J].
Dunbar, J ;
Ticknor, LO ;
Kuske, CR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (01) :190-197
[8]   Changes in the succession and diversity of protozoan and microbial populations in soil spiked with a range of copper concentrations [J].
Ekelund, F ;
Olsson, S ;
Johansen, A .
SOIL BIOLOGY & BIOCHEMISTRY, 2003, 35 (11) :1507-1516
[9]  
Ettler V, 2005, CHEMOSPHERE, V58, P1449, DOI 10.1016/j.chemosphere.2004.09.084
[10]  
Fontaine S, 2004, SOIL SCI SOC AM J, V68, P125, DOI 10.2136/sssaj2004.0125