Development and Validation of RP-HPLC Method for the Determination of Adefovir Dipivoxil in Bulk and in Pharmaceutical Formulation

被引：9

作者：

Ahmed, Zaheer ^{[1
]}

Gopinath, B. ^{[1
]}

Shetty, A. Sathish Kumar ^{[1
]}

Sridhar, B. K. ^{[1
]}

机构：

[1] Natl Coll Pharm, Dept Pharmaceut Anal, Shimoga 577201, KS, India

来源：

E-JOURNAL OF CHEMISTRY | 2009年 / 6卷 / 02期

关键词：

Adefovir dipivoxil; RP-HPLC; Validation;

D O I：

10.1155/2009/157868

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A rapid and sensitive RP-HPLC method with UV detection (262 nm) for routine analysis of adefovir dipivoxil in bulk and in pharmaceutical formulation was developed. Chromatography was performed with mobile phase containing a mixture of acetonitrile and phosphate buffer (50:50, v/v) with flow rate 1.0 mL min(-1). In the range of 5.0-100 mu g/mL, the linearity of adefovir dipivoxil shows a correlation co-efficient of 0.9999. The proposed method was validated by determining sensitivity accuracy, precision, robustness stability, specificity, selectivity and system suitability parameters.

引用

页码：469 / 474

页数：6

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