Transport of live yeast and zebrafish embryo on a droplet ("digital'') microfluidic platform

被引：38
作者：
Son, Sang Uk
Garrell, Robin L. [1 ]
机构：
[1] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
来源：
LAB ON A CHIP | 2009年 / 9卷 / 16期
基金：
美国国家卫生研究院;
关键词：
SYSTEMS; DEVICES; CHIP;
D O I：
10.1039/b906257b
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We demonstrate the first programmed transport of live yeast (Saccharomyces cerevisiae) and a zebrafish embryo (Danio rerio) within droplets in a two-plate digital microfluidic device. The yeast remained viable after transport, and the actuated droplets left no yeast behind. A zebrafish embryo transported 2 hours after fertilization developed normally and hatched. Dechorionation was demonstrated by mixing a droplet of digestive reagent droplet with a droplet containing the embryo. These results demonstrate the potential for using a droplet microfluidic device as an alternative to microwell plates for yeast and zebrafish assays.
引用
收藏
页码：2398 / 2401
页数：4
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