Molecular characterization, expression analysis of Chemerin gene and its potential role in intramuscular adipocyte differentiation of goat

被引：6
作者：
Ma, Jieqiong [1 ,2 ,3 ]
Liao, Honghai [1 ,4 ]
Lin, Yaqiu [2 ,3 ]
Huang, Kai [1 ,2 ,3 ]
Zhu, Jiangjiang [1 ,2 ]
Wang, Yong [1 ,2 ]
机构：
[1] Southwest Minzu Univ, Key Lab Sichuan Prov Qinghai Tibetan Plateau Anim, 4th Sect, Chengdu 610041, Peoples R China
[2] Southwest Minzu Univ, Minist Educ, Key Lab Qinghai Tibetan Plateau Anim Genet Resour, Chengdu 610041, Peoples R China
[3] Southwest Minzu Univ, Coll Life Sci & Technol, Chengdu, Sichuan, Peoples R China
[4] Agr Serv Ctr Panshui Town, Zunyi, Peoples R China
来源：
ANIMAL BIOTECHNOLOGY | 2020年 / 31卷 / 05期
基金：
国家重点研发计划; 中国国家自然科学基金;
关键词：
Goat; Chemerin; intramuscular fat; IMF; differentiation; siRNA; ADIPOSE-TISSUE; RECEPTOR; ADIPOKINE; HEALTH; FAT; ADIPOGENESIS; CLONING;
D O I：
10.1080/10495398.2019.1606818
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Chemerin is a cytokine secreted by adipose cells and plays an important regulatory role in adipocyte differentiation and lipid metabolism. However, the function of Chemerin on intramuscular fat (IMF) deposition is still unknown in goat. Here, we cloned the cDNA of goat Chemerin gene using quantitative real-time PCR (qRT-PCR) technique and further clarified the expression pattern of Chemerin in various tissues of goat. The siRNA against Chemerin were synthesized to explore the potential role in intramuscular preadipocyte differentiation of goat. Our results showed that the full-length open reading frame (ORF) of Chemerin was 489 bp encoding 162 amino acids, mainly expressed in liver and Kidney. The interference of Chemerin significantly inhibited the differentiation of goat intramuscular preadipocyte and down-regulated significantly the expression levels of adipogenic marker genes LPL, AP2, SREBP1, PPAR gamma, C/EBP alpha, and C/EBP beta. These data suggest that Chemerin is a positive regulator of lipogenesis in goat intramuscular preadipocytes. Interestingly, the temporal expression pattern of Chemerin was similar to CMKLR1 during preadipocytre differentiation. These results will facilitate the research on the action of Chemerin gene in goat intramuscular fat deposition.
引用
收藏
页码：382 / 390
页数：9
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