Global Analysis of Predicted G Protein-Coupled Receptor Genes in the Filamentous Fungus, Neurospora crassa

被引：39

作者：

Cabrera, Ilva E. ^{[1
,2
]}

Pacentine, Itallia V. ^{[1
,3
]}

Lim, Andrew ^{[1
,3
]}

Guerrero, Nayeli ^{[1
,3
]}

Krystofova, Svetlana ^{[1
]}

Li, Liande ^{[1
]}

Michkov, Alexander V. ^{[1
,2
]}

Servin, Jacqueline A. ^{[1
]}

Ahrendt, Steven R. ^{[1
,2
]}

Carrillo, Alexander J. ^{[1
,3
]}

Davidson, Liza M. ^{[3
]}

Barsoum, Andrew H. ^{[3
]}

Cao, Jackie ^{[3
]}

Castillo, Ronald ^{[3
]}

Chen, Wan-Ching ^{[3
]}

Dinkchian, Alex ^{[3
]}

Kim, Stephanie ^{[3
]}

Kitada, Sho M. ^{[3
]}

Lai, Taffani H. ^{[3
]}

Mach, Ashley ^{[3
]}

Malekyan, Cristin ^{[3
]}

Moua, Toua R. ^{[3
]}

Torres, Carlos Rojas ^{[3
]}

Yamamoto, Alaina ^{[3
]}

Borkovich, Katherine A. ^{[1
,2
]}

机构：

[1] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA

[2] Univ Calif Riverside, Grad Program Genet Genom & Bioinformat, Riverside, CA 92521 USA

[3] Univ Calif Riverside, Coll Nat & Agr Sci, Riverside, CA 92521 USA

来源：

G3-GENES GENOMES GENETICS | 2015年 / 5卷 / 12期

关键词：

filamentous fungi; G protein-coupled receptors; functional genomics; signal transduction; Heterotrimeric G proteins; HETEROTRIMERIC G-PROTEINS; MATING-TYPE; STRUCTURAL INSIGHTS; PHENOTYPIC ANALYSIS; MAGNAPORTHE-GRISEA; MEMBRANE-PROTEIN; GENOME SEQUENCE; ALPHA-SUBUNITS; DIFFERENTIATION; YEAST;

D O I：

10.1534/g3.115.020974

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

G protein-coupled receptors (GPCRs) regulate facets of growth, development, and environmental sensing in eukaryotes, including filamentous fungi. The largest predicted GPCR class in these organisms is the Pth11-related, with members similar to a protein required for disease in the plant pathogen Magnaporthe oryzae. However, the Pth11-related class has not been functionally studied in any filamentous fungal species. Here, we analyze phenotypes in available mutants for 36 GPCR genes, including 20 Pth11-related, in the model filamentous fungus Neurospora crassa. We also investigate patterns of gene expression for all 43 predicted GPCR genes in available datasets. A total of 17 mutants (47%) possessed at least one growth or developmental phenotype. We identified 18 mutants (56%) with chemical sensitivity or nutritional phenotypes (11 uniquely), bringing the total number of mutants with at least one defect to 28 (78%), including 15 mutants (75%) in the Pth11-related class. Gene expression trends for GPCR genes correlated with the phenotypes observed for many mutants and also suggested overlapping functions for several groups of co-transcribed genes. Several members of the Pth11-related class have phenotypes and/or are differentially expressed on cellulose, suggesting a possible role for this gene family in plant cell wall sensing or utilization.

引用

页码：2729 / 2743

页数：15

共 91 条

[1] Global Survey of Canonical Aspergillus flavus G Protein-Coupled Receptors [J].

Affeldt, Katharyn J. ;

Carrig, Joseph ;

Amare, Meareg ;

Keller, Nancy P. .

MBIO, 2014, 5 (05)

[2]

[Anonymous], 2014, R: a Language and Environment for Statistical Computing

[3] FINGERPRINTING G-PROTEIN-COUPLED RECEPTORS [J].

ATTWOOD, TK ;

FINDLAY, JBC .

PROTEIN ENGINEERING, 1994, 7 (02) :195-203

[4]

Baasiri RA, 1997, GENETICS, V147, P137

[5] The circadian clock of Neurospora crassa [J].

Baker, Christopher L. ;

Loros, Jennifer J. ;

Dunlap, Jay C. .

FEMS MICROBIOLOGY REVIEWS, 2012, 36 (01) :95-110

[6] The band mutation in Neurospora crassa is a dominant allele of ras-1 implicating RAS signaling in circadian output [J].

Belden, William J. ;

Larrondo, Luis F. ;

Froehlich, Allan C. ;

Shi, Mi ;

Chen, Chen-Hui ;

Loros, Jennifer J. ;

Dunlap, Jay C. .

GENES & DEVELOPMENT, 2007, 21 (12) :1494-1505

[7] Actin organization and dynamics in filamentous fungi [J].

Berepiki, Adokiye ;

Lichius, Alexander ;

Read, Nick D. .

NATURE REVIEWS MICROBIOLOGY, 2011, 9 (12) :876-887

[8] The nop-1 gene of Neurospora crassa encodes a seven transmembrane helix retinal-binding protein homologous to archaeal rhodopsins [J].

Bieszke, JA ;

Braun, EL ;

Bean, LE ;

Kang, SC ;

Natvig, DO ;

Borkovich, KA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (14) :8034-8039

[9] A eukaryotic protein, NOP-1, binds retinal to form an archaeal rhodopsin-like photochemically reactive pigment [J].

Bieszke, JA ;

Spudich, EN ;

Scott, KL ;

Borkovich, KA ;

Spudich, JL .

BIOCHEMISTRY, 1999, 38 (43) :14138-14145

[10] The fungal opsin gene nop-1 is negatively-regulated by a component of the blue light sensing pathway and influences conidiation-specific gene expression in Neurospora crassa [J].

Bieszke, Jennifer A. ;

Li, Liande ;

Borkovich, Katherine A. .

CURRENT GENETICS, 2007, 52 (3-4) :149-157

← 1 2 3 4 5 6 7 8 9 10 →