Efficiency of Histidine Rich Protein II-Based Rapid Diagnostic Tests for Monitoring Malaria Transmission Intensities in an Endemic Area

被引:2
作者
Modupe, Dokunmu Titilope [1 ]
Iyabo, Olasehinde Grace [2 ]
Oladoke, Oladejo David [1 ]
Oladeji, Olanrewaju [1 ]
Abisola, Akinbobola [1 ]
Ufuoma, Adjekukor Cynthia [1 ]
Faith, Yakubu Omolara [1 ]
Humphrey, Adebayo Abiodun [1 ]
机构
[1] Covenant Univ, Dept Biochem, Ota, Ogun State, Nigeria
[2] Covenant Univ, Biol Sci Dept, Ota, Ogun State, Nigeria
来源
PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON APPLIED SCIENCES (ICAS-2) | 2018年 / 1954卷
关键词
PLASMODIUM-FALCIPARUM MALARIA; GAMETOCYTE CARRIAGE; ASYMPTOMATIC MALARIA; NIGERIAN CHILDREN; PRIMAQUINE; FIELD; PERFORMANCE; INFECTIONS; COMMUNITY; EFFICACY;
D O I
10.1063/1.5033381
中图分类号
O59 [应用物理学];
学科分类号
摘要
In recent years there has been a global decrease in the prevalence of malaria due to scaling up of control measures, hence global control efforts now target elimination and eradication of the disease. However, a major problem associated with elimination is asymptomatic reservoir of infection especially in endemic areas. This study aims to determine the efficiency of histidine rich protein II (HRP-2) based rapid diagnostic tests (RDT) for monitoring transmission intensities in an endemic community in Nigeria during the pre-elimination stage. Plasmodium falciparum asymptomatic malaria infection in healthy individuals and symptomatic cases were detected using HRP-2. RDT negative tests were re-checked by microscopy and by primer specific PCR amplification of merozoite surface protein 2 (msp-2) for asexual parasites and Pfs25 gene for gametocytes in selected samples to detect low level parasitemia undetectable by microscopy. The mean age of the study population (n=280) was 6.12 years [95% CI 5.16 - 7.08, range 0.5 - 55], parasite prevalence was 44.6% and 36.3% by microscopy and RDT respectively (p = 0.056). The parasite prevalence of 61.5% in children aged >2 - 10 years was significantly higher than 3.7% rate in adults > 18years (p < 0.0001, chi(2) = 60.45). RDT detected additional 29.6% asymptomatic cases but a lower specificity of 68.8% in symptomatic carriers. In 15 selected RDT positive samples, only 6 were positive by PCR and no gametocyte was detected. The results indicate that HRP-2 RDTs are a vital tool for understanding transmission dynamics and detecting immune-suppressed, recent and asymptomatic infections, thus crucial to tackle low level transmission and eliminating malaria in endemic areas.
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