ENHANCED PLANT REGENERATION IN LEMNA MINOR BY AMINO ACIDS

被引：0

作者：

Yang, Lin ^{[1
,2
]}

Han, Huajun ^{[1
]}

Zuo, Zhaojiang ^{[1
]}

Zhou, Kaiqiang ^{[1
]}

Ren, Cong ^{[1
]}

Zhu, Yerong ^{[1
]}

Bai, Yanling ^{[1
]}

Yongwang ^{[1
]}

机构：

[1] Nankai Univ, Coll Life Sci, Dept Plant Biol & Ecol, Tianjin 300071, Peoples R China

[2] Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China

来源：

PAKISTAN JOURNAL OF BOTANY | 2014年 / 46卷 / 03期

基金：

中国国家自然科学基金;

关键词：

SUSPENSION-CULTURES; CALLUS INDUCTION; SOMATIC EMBRYOGENESIS; CARROT TISSUE; SPIRODELA; GROWTH; NITROGEN; DUCKWEED; PROLINE; WATER;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In present study we investigated the effects of different L-amino acids on the plant regeneration from callus of Lemna minor, and established an efficient protocol. Among the 20 L-amino acids, only L-Ser and L-Gly showed significant improving effect, with the optimal concentration being 1 mM and 1.5 mM, respectively. A regeneration frequency of 46% was observed when the callus transferred to the regeneration medium with addition of 1 mM L-Ser for 11 days. After 26 days of cultivation, the frond regeneration achieved 100% and 94% for 1 mM L-Ser and 1.5 mM L-Gly treatment, respectively.

引用

页码：939 / 943

页数：5

共 32 条

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