ENHANCED PLANT REGENERATION IN LEMNA MINOR BY AMINO ACIDS

被引：0

作者：

Yang, Lin ^{[1
,2
]}

Han, Huajun ^{[1
]}

Zuo, Zhaojiang ^{[1
]}

Zhou, Kaiqiang ^{[1
]}

Ren, Cong ^{[1
]}

Zhu, Yerong ^{[1
]}

Bai, Yanling ^{[1
]}

Yongwang ^{[1
]}

机构：

[1] Nankai Univ, Coll Life Sci, Dept Plant Biol & Ecol, Tianjin 300071, Peoples R China

[2] Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China

来源：

PAKISTAN JOURNAL OF BOTANY | 2014年 / 46卷 / 03期

基金：

中国国家自然科学基金;

关键词：

SUSPENSION-CULTURES; CALLUS INDUCTION; SOMATIC EMBRYOGENESIS; CARROT TISSUE; SPIRODELA; GROWTH; NITROGEN; DUCKWEED; PROLINE; WATER;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In present study we investigated the effects of different L-amino acids on the plant regeneration from callus of Lemna minor, and established an efficient protocol. Among the 20 L-amino acids, only L-Ser and L-Gly showed significant improving effect, with the optimal concentration being 1 mM and 1.5 mM, respectively. A regeneration frequency of 46% was observed when the callus transferred to the regeneration medium with addition of 1 mM L-Ser for 11 days. After 26 days of cultivation, the frond regeneration achieved 100% and 94% for 1 mM L-Ser and 1.5 mM L-Gly treatment, respectively.

引用

页码：939 / 943

页数：5

共 32 条

[1]

Bertomeu M.J., 2010, PLANT SIGNAL BEHAV, V5, P67

[2]

Bertomeu M.J., 2010, J EXP BOT, V62, P1229

[3]

Chang S. M., 1977, B I CHEM ACAD SIN, V24, P19

[4] REGENERATION OF LEMNA-GIBBA G-3 THROUGH CALLUS-CULTURE [J].

CHANG, WC ;

CHIU, PL .

ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE, 1978, 89 (01) :91-94

[5] Genetic transformation of Indian isolate of Lemna minor mediated by Agrobacterium tumefaciens and recovery of transgenic plants [J].

Chhabra G. ;

Chaudhary D. ;

Sainger M. ;

Jaiwal P.K. .

Physiology and Molecular Biology of Plants, 2011, 17 (2) :129-136

[6] CALLOGENESIS AND CARBOHYDRATE UTILIZATION IN LEMNA-MINOR [J].

FRICK, H .

JOURNAL OF PLANT PHYSIOLOGY, 1991, 137 (04) :397-401

[7] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS [J].

GAMBORG, OL ;

MILLER, RA ;

OJIMA, K .

EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) :151-+

[8] Molecular biology of the plastidic phosphorylated serine biosynthetic pathway in Arabidopsis thaliana [J].

Ho, CL ;

Saito, K .

AMINO ACIDS, 2001, 20 (03) :243-259

[9] Embryogenic callus formation, growth and regeneration in callus and suspension cultures of Miscanthus x ogiformis Honda Giganteus' as affected by proline [J].

Holme, IB ;

Krogstrup, P ;

Hansen, J .

PLANT CELL TISSUE AND ORGAN CULTURE, 1997, 50 (03) :203-210

[10]

Iram S, 2012, PAK J BOT, V44, P553

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