Structural studies of the spliceosome: zooming into the heart of the machine

被引:44
作者
Galej, Wojciech P. [1 ]
Thi Hoang Duong Nguyen [1 ]
Newman, Andrew J. [1 ]
Nagai, Kiyoshi [1 ]
机构
[1] MRC, Mol Biol Lab, Cambridge CB2 0QH, England
基金
英国医学研究理事会;
关键词
SPLICING FACTOR PRP8; METAL-ION COORDINATION; CRYSTAL-STRUCTURE; U5; SNRNP; RNA INTERACTIONS; CATALYTIC CORE; PROTEIN; DOMAIN; REVEALS; CWC2;
D O I
10.1016/j.sbi.2013.12.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Spliceosomes are large, dynamic ribonucleoprotein complexes that catalyse the removal of introns from messenger RNA precursors via a two-step splicing reaction. The recent crystal structure of Prp8 has revealed Reverse Transcriptase-like, Linker and Endonuclease-like domains. The intron branchpoint cross-link with the Linker domain of Prp8 in active spliceosomes and together with suppressors of 5' and 3' splice site mutations this unambiguously locates the active site cavity. Structural and mechanistic similarities with group II selfsplicing introns have encouraged the notion that the spliceosome is at heart a ribozyme, and recently the ligands for two catalytic magnesium ions were identified within U6 snRNA. They position catalytic divalent metal ions in the same way as Domain V of group ll intron RNA, suggesting that the spliceosome and group II intron use the same catalytic mechanisms.
引用
收藏
页码:57 / 66
页数:10
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