The antimicrobial action of histones in the reproductive tract of cow

被引：14

作者：

Drab, Tomas ^{[1
]}

Kracmerova, Jana ^{[1
]}

Hanzlikova, Eva ^{[1
]}

Cerna, Tereza ^{[1
]}

Litvakova, Rozalie ^{[1
]}

Pohlova, Alzbeta ^{[1
]}

Ticha, Marie ^{[1
]}

Prikryl, Petr ^{[2
,3
]}

Liberda, Jiri ^{[1
]}

机构：

[1] Charles Univ Prague, Dept Biochem, Fac Sci, Prague 12843, Czech Republic

[2] Charles Univ Prague, Fac Med 1, Inst Pathophysiol, Prague 12853 2, Czech Republic

[3] Charles Univ Prague, Fac Med 1, CEH, Prague 12853 2, Czech Republic

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 443卷 / 03期

关键词：

Bovine reproductive tract; Extracellular histones; Histone-like proteins; Antimicrobial properties; LEUKOCYTE PROTEASE INHIBITOR; H1; PROTEINS; PEPTIDE; BINDING; EXPRESSION; INFECTION; GENE; SKIN; DNA;

D O I：

10.1016/j.bbrc.2013.12.077

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An infection of any part of female reproductive tract can severely interfere with fertility and reproduction. The fluids and epithelium from the lumen of the female reproductive tract (uterus, oviduct and ovarian follicle) are a known source of antimicrobial action in several species. In this study, we compared the antimicrobial properties of fluids from the reproductive tract of a cow. After removal of small molecules, we demonstrated that there is an antimicrobial activity connected with a fraction of compounds with a molecular mass range between 3500 and 30,000. The most probable candidates responsible for the observed antimicrobial effect were subsequently identified by mass spectroscopy as histones H2A type 2-C, H2B type 1-K, H3.3, and H4. The antimicrobial role of histone H2B was further confirmed by using an antibody against this histone. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：987 / 990

页数：4

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