Chronic Arsenic Exposure Impairs Macrophage Functions in the Exposed Individuals

被引：70

作者：

Banerjee, Nilanjana ^{[1
]}

Banerjee, Saptarshi ^{[2
]}

Sen, Rupashree ^{[3
]}

Bandyopadhyay, Apurba ^{[1
]}

Sarma, Nilendu ^{[4
]}

Majumder, Papiya ^{[5
]}

Das, Jayanta K. ^{[6
]}

Chatterjee, Mitali ^{[3
]}

Kabir, Syed N. ^{[7
]}

Giri, Ashok K. ^{[1
]}

机构：

[1] Indian Inst Chem Biol, Mol & Human Genet Div, Kolkata 700032, India

[2] Ramkrishna Mission Seva Pratisthan, Dept Ophthalmol, Kolkata, India

[3] Inst Post Grad Med Educ & Res, Dept Pharmacol, Kolkata 700020, India

[4] Sir Nil Ratan Sircar Med Coll & Hosp, Kolkata, India

[5] Peerless Hosp & BC Roy Res Ctr, Kolkata, India

[6] W Bank Hosp, Dept Dermatol, Howrah 711109, India

[7] Indian Inst Chem Biol, Cell Biol & Physiol Div, Kolkata 700032, India

来源：

JOURNAL OF CLINICAL IMMUNOLOGY | 2009年 / 29卷 / 05期

关键词：

Arsenic; cell adhesion; macrophage; phagocytosis; Rho A-ROCK pathway; WEST-BENGAL; DRINKING-WATER; CYTOKINE SECRETION; INORGANIC ARSENITE; GENE-EXPRESSION; GROWTH-FACTOR; SKIN-LESIONS; CELLS; DIFFERENTIATION; APOPTOSIS;

D O I：

10.1007/s10875-009-9304-x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Owing to the established roles of human macrophages in immune defense, we investigated the effect of chronic arsenic exposure upon these major hematopoietic cells in 70 arsenic-exposed individuals with skin lesions and 64 unexposed individuals. Human monocyte-derived macrophages were prepared from peripheral blood mononuclear cells, by culture of the adherent cells for 6 days in medium supplemented with granulocyte-monocyte colony stimulating factor. Parameters studied included cell adhesion capacity, expression of CD54 and F-actin, nitric oxide production, phagocytic capacity, and effect of arsenic on Rho A-ROCK pathway. In macrophages of exposed individuals when compared to unexposed group, there was cell rounding accompanied with a significant (p < 0.001) loss of cell adhesion capacity, decrease in nitric oxide production, impaired phagocytic capacity, and decreased CD 54 and F-actin expression. Additionally, chronic arsenic exposure affected Rho A-ROCK pathway which in turn impaired macrophage functions. These altogether could contribute significantly to arsenic-induced immunosuppression observed in the arsenic-exposed individuals.

引用

页码：582 / 594

页数：13

共 46 条

[1] Functional heterogeneity of colony-stimulating factor-induced human monocyte-derived macrophages [J].

Akagawa, KS .

INTERNATIONAL JOURNAL OF HEMATOLOGY, 2002, 76 (01) :27-34

[2]

Allen WE, 1997, J CELL SCI, V110, P707

[3] Cancer incidence after immunosuppressive treatment following kidney transplantation [J].

Andrés, A .

CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY, 2005, 56 (01) :71-85

[4] Genomic and proteomic profiling of responses to toxic metals in human lung cells [J].

Andrew, AS ;

Warren, AJ ;

Barchowsky, A ;

Temple, KA ;

Klei, L ;

Soucy, NV ;

O'Hara, KA ;

Hamilton, JW .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2003, 111 (06) :825-838

[5]

[Anonymous], 1999, Arsenic in drinking water

[6]

[Anonymous], 1996, Guidelines for drinking-water quality, V2, P940

[7]

ATSDR, 1999, TOX PROF ARS

[8] Polymorphism in the ERCC2 codon 751 is associated with arsenic-induced premalignant hyperkeratosis and significant chromosome aberrations [J].

Banerjee, Mayukh ;

Sarkar, Jyotirmoy ;

Das, Jayanta K. ;

Mukherjee, Angshuman ;

Sarkar, Ajoy K. ;

Mondal, Lakshmikanta ;

Giri, Ashok K. .

CARCINOGENESIS, 2007, 28 (03) :672-676

[9] Arsenic-induced mitochondrial instability leading to programmed cell death in the exposed individuals [J].

Banerjee, Nilanjana ;

Banerjee, Mayukh ;

Ganguly, Sudipto ;

Bandyopadhyay, Santu ;

Das, Jayanta K. ;

Bandyopadhay, Apurba ;

Chatterjee, Mitali ;

Giri, Ashok K. .

TOXICOLOGY, 2008, 246 (2-3) :101-111

[10]

Basu A, 2004, CANCER EPIDEM BIOMAR, V13, P820

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