Detection of Helicobacter pylori in water by direct PCR

被引：30

作者：

Benson, JA ^{[1
]}

Fode-Vaughan, KA ^{[1
]}

Collins, MLP ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53201 USA

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2004年 / 39卷 / 03期

关键词：

detection; direct PCR; DPCR; Helicobacter pylori; PCR;

D O I：

10.1111/j.1472-765X.2004.01555.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: This paper demonstrates a rapid, simple method for the detection of Helicobacter pylori in water that eliminates the need for recovery of cells or DNA extraction prior to PCR. Methods and Results: Direct polymerase chain reaction (DPCR) with primers specific for H. pylori ureA (urease, subunit A) were used to detect H. pylori added to groundwater. DPCR also detected H. pylori in a naturally contaminated water sample. Conclusions: DPCR should provide an improved method to assess contamination of water by H. pylori. Significance and Impact of the Study: This simple, rapid method for detection of H. pylori in water will provide an improved means to investigate the possible role of water as a disease vector.

引用

页码：221 / 225

页数：5

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