Simultaneous HPLC determination of gamma amino butyric acid (GABA) and lysine in selected Pakistani rice varieties by pre-column derivatization with 2-Hydroxynaphthaldehyde

被引：36

作者：

Hayat, Amir ^{[1
]}

Jahangir, Taj Muhammad ^{[1
]}

Khuhawar, Muhammad Yar ^{[1
]}

Alamgir, Malik ^{[1
]}

Siddiqui, Amna Jabbar ^{[2
]}

Musharraf, Syed Ghulam ^{[2
]}

机构：

[1] Univ Sindh, Inst Adv Res Studies Chem Sci, Jamshoro 76062, Pakistan

[2] Univ Karachi, Int Ctr Chem & Biol Sci, HEJ Res Inst Chem, Karachi 75270, Pakistan

来源：

JOURNAL OF CEREAL SCIENCE | 2014年 / 60卷 / 02期

关键词：

GABA; Lysine; HPLC; Rice; PERFORMANCE LIQUID-CHROMATOGRAPHY; AMINOBUTYRIC-ACID; GLUTAMATE; MICRODIALYSIS; TAURINE; FLUORESCENCE; ASPARTATE; GLYCINE; RAT;

D O I：

10.1016/j.jcs.2014.05.011

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A selective and sensitive HPLC method for the simultaneous determination of gamma amino butyric acid (GABA) and lysine in Pakistani rice varieties was developed. Both analytes were detected in rice varieties as Schiff base derivatives with 2-hydroxynaphthaldehyde. The derivatives were analyzed on a reverse phase C-18 column with Diode Array Detector (DAD) at 254 nm. The calibration curves were found linear over a concentration range of 3.83-34.58 mu g/mL for GABA and 5.16-48.68 mu g/mL for lysine with a correlation coefficient of 0.998 for both standards. GABA and lysine contents were found higher in brown rice varieties (4.1-6.58 mg/100 g for GABA and 15.1-27.6 mg/100 g for lysine) than the polished varieties (0.32-0.47 mg/100 g GABA and 13.1-19.8 mg/100 g lysine). This method could be used for separation and quantification of GABA and lysine simultaneously in food samples, especially in cereal seeds. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：356 / 360

页数：5