IL-12p40 gene expression in lung and hilar lymph nodes of MPS-resistant pigs

被引：1

作者：

Sakuma, Akiko ^{[1
,2
,3
]}

Sugawara, Shizuka ^{[3
]}

Hidaka, Hikaru ^{[3
]}

Nakajo, Mitsuru ^{[1
]}

Suda, Yoshihito ^{[4
]}

Shimazu, Tomoyuki ^{[4
]}

Rose, Michael T. ^{[5
]}

Urakawa, Megumi ^{[3
,6
]}

Zhuang, Tao ^{[3
,6
]}

Zhao, Guoqi ^{[7
]}

Watanabe, Kouichi ^{[3
,6
]}

Nochi, Tomonori ^{[3
,6
]}

Kitazawa, Haruki ^{[3
,6
]}

Katoh, Kazuo ^{[3
]}

Suzuki, Keiichi ^{[3
]}

Aso, Hisashi ^{[3
,6
]}

机构：

[1] Miyagi Livestock Expt Stn, Osaki, Japan

[2] Miyagi Prefectural Sendai Livestock Hyg Serv Ctr, Sendai, Miyagi, Japan

[3] Tohoku Univ, Grad Sch Agr Sci, Int Educ & Res Ctr Food & Agr Immunol CFAI, Sendai, Miyagi, Japan

[4] Miyagi Univ, Dept Food Agr & Environm, Sendai, Miyagi, Japan

[5] Univ Tasmania, Tasmanian Inst Agr, Sandy Bay, Tas, Australia

[6] Tohoku Univ, Grad Sch Agr Sci, Lab Anim Hlth Sci, Sendai, Miyagi, Japan

[7] Yangzhou Univ, Coll Anim Sci & Technol, Inst Anim Culture Collect & Applicat, Yangzhou, Jiangsu, Peoples R China

来源：

ANIMAL SCIENCE JOURNAL | 2020年 / 91卷 / 01期

关键词：

cytokine; disease resistance; histopathology; landrace pigs; Mycoplasma hyopneumoniae; RESPIRATORY SYNDROME VIRUS; MYCOPLASMA-HYOPNEUMONIAE; LANDRACE PIGS; SWINE MPS; INTERLEUKIN-12; INFECTION; PNEUMONIA; CYTOKINES; DISEASES; IL-18;

D O I：

10.1111/asj.13450

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Mycoplasma pneumonia of swine (MPS) is caused by Mycoplasma hyopneumoniae (M.hp) and is a common chronic respiratory disease of pigs. Recently, a genetically selected variant of the Landrace pig (Miyagino L2) has a lower incidence of pulmonary MPS lesions. We investigated the pathological and immunological characteristics of MPS resistance in these pigs (n = 24) by comparing with the normal landrace pig (control: n = 24). The pathological MPS lung lesion score in MPS-selected landrace pigs was significantly lower than in the control. The gene expression of interleukin (IL)-12p40, which acts as a chemoattractant and a component of the bioactive cytokines IL-12 and IL-23, was significantly higher at the hilar lymph nodes, lung, and spleen in MPS-selected landrace pigs than in control landrace pigs, and these were negatively correlated with the macroscopic MPS lung lesion score. In summary, we demonstrate that resistance against MPS in Miyagino L2 pigs is associated with IL-12p40 up-regulation, in comparison with normal landrace pigs without the MPS vaccine. In addition, a comparative study of macroscopic MPS lung lesions and IL-12p40 gene expression in lung and hilar lymph nodes may lead to beneficial selection traits for the genetic selection for MPS resistance in pigs.

引用

页数：11

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