Pseudomonas aeruginosa injects NDK into host cells through a type III secretion system

被引:31
作者
Neeld, Dennis [1 ]
Jin, Yongxin [2 ]
Bichsel, Candace [1 ]
Jia, Jinghua [1 ]
Guo, Jianhui [2 ]
Bai, Fang [2 ]
Wu, Weihui [2 ]
Ha, Un-Hwan [3 ]
Terada, Naohiro [4 ]
Jin, Shouguang [1 ]
机构
[1] Univ Florida, Dept Mol Genet & Microbiol, Gainesville, FL 32611 USA
[2] Nankai Univ, Coll Life Sci, Dept Microbiol, Tianjin 300071, Peoples R China
[3] Korea Univ, Dept Biotechnol & Bioinformat, Sejong, South Korea
[4] Univ Florida, Dept Pathol, Gainesville, FL 32611 USA
来源
MICROBIOLOGY-SGM | 2014年 / 160卷
基金
新加坡国家研究基金会; 美国国家科学基金会; 美国国家卫生研究院;
关键词
NUCLEOSIDE-DIPHOSPHATE-KINASE; EFFECTORS; TRANSLOCATION; INVOLVEMENT; INFECTION; DELIVERY; PROTEIN; GENES;
D O I
10.1099/mic.0.078139-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pseudomonas aeruginosa is a Gram-negative opportunistic human pathogen possessing a type III secretion system (T3SS) which injects toxic effector proteins into mammalian host cells. In previous studies, P. aeruginosa strains lacking all of the known type III effectors were shown to cause cytotoxicity upon prolonged infection time. In this study, we report the identification of a new cytotoxin, nucleoside diphosphate kinase (NDK), which is injected into eukaryotic cells in a T3SS-dependent manner. Injection of NDK is inhibited by the presence of previously known effectors of the T3SS, with an effectorless strain injecting the highest amount, suggesting active competition with the known T3SS effectors. NDK is shown to cause a cytotoxic response when expressed in eukaryotic cells, and P. aeruginosa strains harbouring NDK also show a greater toxicity than strains lacking it. Interestingly, the cytotoxic effect of intracellular NDK is independent of its kinase activity. In previous studies, NDK was shown to be secreted into culture supernatants via a type I secretion system and cause cytotoxicity in a kinase-dependent manner. Therefore, the current study highlights an alternative route of NDK secretion as well as two different cytotoxic mechanisms of NDK, depending on the extra- or intra-cellular location of the protein.
引用
收藏
页码:1417 / 1426
页数:10
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