Sample pretreatment on a microchip with an integrated electrospray emitter

被引:16
作者
Lindberg, Peter
Dahlin, Andreas P.
Bergstrom, Sara K.
Thorslund, Sara
Andren, Per E.
Nikolajeff, Fredrik
Bergquist, Jonas
机构
[1] Uppsala Univ, Dept Analyt Chem, S-75124 Uppsala, Sweden
[2] Uppsala Univ, Dept Engn Sci, S-75124 Uppsala, Sweden
[3] Uppsala Univ, Lab Biol & Med Mass Spectrometry, S-75124 Uppsala, Sweden
[4] Uppsala Univ, Dept Pharmaceut Biosci, S-75124 Uppsala, Sweden
关键词
electrospray emitter; microchip; PDMS; sample pretreatment;
D O I
10.1002/elps.200500763
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This study presents a microbead-packed PDMS microchip with an integrated electrospray emitter for sample pretreatment prior to sheathless ESI-MS. We prove the concept of analytical functions integrated onto a cm-sized area of a single bulk material. The microchip consists of two PDMS substrates replicated from SU-8 fabricated silicon wafer masters, bonded together after oxidation by corona discharge treatment. The channel within the microchip contains a grid structure that was used to trap 5 mu m hypercross-linked polystyrene beads. The beads acted as a medium for sample clesalting and enrichment. Electrical contact for the sheathless ESI process was achieved by coating the integrated emitter with conductive graphite powder after applying a thin layer of PDMS as glue. The coating as well as the bond of the PDMS structures showed excellent durability. A continuous spray was obtained from the microchip for over 800 h in a long-term electrospray stability experiment. Desalting and enrichment of neuropeptides from a physiological salt solution was successful by loading the sample onto the packed beads, followed by a washing and an eluting step. The results were obtained and evaluated using a TOF MS. An LOD of approximately 20 fmol (loaded onto the beads) for angiotensin 11 was obtained from a sample of neuropeptides dissolved in physiological salt solution.
引用
收藏
页码:2075 / 2082
页数:8
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