Selenomethionine Alleviates AFB1-Induced Damage in Primary Chicken Hepatocytes by Inhibiting CYP450 1A5 Expression via Upregulated SelW Expression

被引:36
作者
Chen, Xingxiang [1 ]
Che, Chaoping [1 ]
Korolchuk, Viktor I. [2 ]
Gan, Fang [1 ]
Pant, Cuiling [1 ]
Huang, Kehe [1 ]
机构
[1] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China
[2] Newcastle Univ, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE4 5PL, Tyne & Wear, England
基金
中国国家自然科学基金;
关键词
selenomethionine; AFB1; cytochrome P450 enzymes; siRNA; selenoprotein W; primary chicken hepatocytes; CELL-CYCLE PROGRESSION; HUMAN CYTOCHROMES P450; AFLATOXIN B-1; SELENOPROTEIN-W; DIETARY SELENIUM; TURKEY LIVER; COVALENT BINDING; OXIDATIVE STRESS; SKELETAL-MUSCLE; VITAMIN-E;
D O I
10.1021/acs.jafc.6b05308
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
This study aims to evaluate the protective effects of selenomethionine (SeMet) on aflatoxin B1 (AFB1)-induced hepatotoxicity in primary chicken hepatocytes. Cell-viability and lactic dehydrogenase activity assays revealed the dose dependence of AFB1 toxicity to chicken hepatocytes. AFB1 concentrations of >.05 mu g/mL significantly reduced glutathione and total superoxide dismutase levels and increased the malondialdehyde concentration and cytochrome P450 enzyme 1A5 (CYP450 1A5) mRNA levels (P < 0.05). AFB1, however; did not affect CYP450 3A37 mRNA levels. Supplementation with 2 mu M SeMet protected against AFB1 induced changes and significantly increased selenoprotein W (SelW) mRNA levels (P < 0.05). Additionally, SelW knockdown attenuated the protective effect of SeMet on AFB1-induced damage and significantly increased the level of CYP450 1A5 expression (P < 0.05). Therefore, SeMet alleviates AFB1-induced damage in priinary chicken hepatocytes by improving SelW expression, thus inhibiting CYP450 1A5 expression.
引用
收藏
页码:2495 / 2502
页数:8
相关论文
共 55 条
[1]   NEW METABOLIC ROLES FOR SELENIUM [J].
ARTHUR, JR ;
BECKETT, GJ .
PROCEEDINGS OF THE NUTRITION SOCIETY, 1994, 53 (03) :615-624
[2]   Mycotoxins [J].
Bennett, JW ;
Klich, M .
CLINICAL MICROBIOLOGY REVIEWS, 2003, 16 (03) :497-+
[3]   Cytotoxicity of occupationally and environmentally relevant mycotoxins [J].
Bünger, J ;
Westphal, G ;
Mönnich, A ;
Hinnendahl, B ;
Hallier, E ;
Müller, M .
TOXICOLOGY, 2004, 202 (03) :199-211
[4]   Novel aflatoxin derivatives and protein conjugates [J].
Cervino, Christian ;
Knopp, Dietmar ;
Weller, Michael G. ;
Niessner, Reinhard .
MOLECULES, 2007, 12 (03) :641-653
[5]   EFFECTS OF DIETARY SELENIUM AND VITAMIN-E ON HEPATIC MIXED-FUNCTION OXIDASE ACTIVITIES AND INVIVO COVALENT BINDING OF AFLATOXIN-B1 IN RATS [J].
CHEN, J ;
GOETCHIUS, MP ;
CAMPBELL, TC ;
COMBS, GF .
JOURNAL OF NUTRITION, 1982, 112 (02) :324-331
[6]   EFFECTS OF DIETARY SELENIUM AND VITAMIN-E ON COVALENT BINDING OF AFLATOXIN TO CHICK LIVER-CELL MACROMOLECULES [J].
CHEN, J ;
GOETCHIUS, MP ;
COMBS, GF ;
CAMPBELL, TC .
JOURNAL OF NUTRITION, 1982, 112 (02) :350-355
[8]  
DAVILA JC, 1983, AM J VET RES, V44, P1877
[9]   Cytochrome P450 enzymes involved in the metabolism of aflatoxin B1 in chickens and quail [J].
Diaz, G. J. ;
Murcia, H. W. ;
Cepeda, S. M. .
POULTRY SCIENCE, 2010, 89 (11) :2461-2469
[10]   SelT, SelW, SelH, and Rdx12: Genomics and molecular insights into the functions of selenoproteins of a novel thioredoxin-like family [J].
Dikiy, Alexander ;
Novoselov, Sergey V. ;
Fomenko, Dmitri E. ;
Sengupta, Aniruddha ;
Carlson, Bradley A. ;
Cerny, Ronald L. ;
Ginalski, Krzysztof ;
Grishin, Nick V. ;
Hatfield, Dolph L. ;
Gladyshev, Vadim N. .
BIOCHEMISTRY, 2007, 46 (23) :6871-6882