This study aims to evaluate the protective effects of selenomethionine (SeMet) on aflatoxin B1 (AFB1)-induced hepatotoxicity in primary chicken hepatocytes. Cell-viability and lactic dehydrogenase activity assays revealed the dose dependence of AFB1 toxicity to chicken hepatocytes. AFB1 concentrations of >.05 mu g/mL significantly reduced glutathione and total superoxide dismutase levels and increased the malondialdehyde concentration and cytochrome P450 enzyme 1A5 (CYP450 1A5) mRNA levels (P < 0.05). AFB1, however; did not affect CYP450 3A37 mRNA levels. Supplementation with 2 mu M SeMet protected against AFB1 induced changes and significantly increased selenoprotein W (SelW) mRNA levels (P < 0.05). Additionally, SelW knockdown attenuated the protective effect of SeMet on AFB1-induced damage and significantly increased the level of CYP450 1A5 expression (P < 0.05). Therefore, SeMet alleviates AFB1-induced damage in priinary chicken hepatocytes by improving SelW expression, thus inhibiting CYP450 1A5 expression.
机构:
Univ Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, ColombiaUniv Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, Colombia
Diaz, G. J.
;
Murcia, H. W.
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Univ Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, ColombiaUniv Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, Colombia
Murcia, H. W.
;
Cepeda, S. M.
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h-index: 0
机构:
Univ Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, ColombiaUniv Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, Colombia
机构:
Univ Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, ColombiaUniv Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, Colombia
Diaz, G. J.
;
Murcia, H. W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, ColombiaUniv Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, Colombia
Murcia, H. W.
;
Cepeda, S. M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, ColombiaUniv Nacl Colombia, Toxicol Lab, Fac Med Vet & Zootecnia, Bogota, DC, Colombia