Absolute brightness of fluorescent microspheres

被引：6

作者：

Finger, Isaac ^{[1
]}

Phillips, Scott ^{[2
]}

Mobley, Elizabeth ^{[1
]}

Tucker, Robert ^{[1
]}

Hess, Henry ^{[1
]}

机构：

[1] Univ Florida, Dept Mat Sci & Engn, Gainesville, FL 32611 USA

[2] ANDOR Technol PLC, S Windsor, CT USA

来源：

LAB ON A CHIP | 2009年 / 9卷 / 03期

关键词：

BIOMOLECULAR MOTOR-DRIVEN; ENGINEERED VIRUS; LIDAR SYSTEM; INTENSITY; PARTICLES; FLUOROPHORE; ASSIGNMENT; NANOSCALE; TRANSPORT; SCAFFOLD;

D O I：

10.1039/b810219h

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The absolute brightness of fluorescent particles, such as dye-containing nano-and microspheres or quantum dots, is a critical design parameter for many applications relying on fluorescence detection. The absolute brightness, defined as the ratio of radiant intensity of emission to illumination intensity of excitation, of nile-red fluorescent microspheres with a 1 micrometre diameter is measured to be 4.2 +/- 1 X 10(-16) m(2)/sr, and the implications for the design of kinesin motor protein-powered "smart dust'' devices and the remote detection of fluorescence are discussed.

引用

页码：476 / 478

页数：3

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