cDNA cloning and characterization of rat C5a anaphylatoxin receptor

被引：23

作者：

Akatsu, H

Miwa, T

Sakurada, C

Fukuoka, Y

Ember, JA

Yamamoto, T

Hugli, TE

Okada, H

机构：

[1] NAGOYA CITY UNIV, SCH MED, DEPT MOL BIOL, MIZUHO KU, NAGOYA, AICHI 467, JAPAN

[2] FUKUSHIMURA HOSP, CHOJU MED INST, TOYOHASHI, AICHI 441, JAPAN

[3] DAIICHI COLL PHARMACEUT SCI, DEPT BIOCHEM, FUKUOKA 815, JAPAN

[4] Scripps Res Inst, DEPT IMMUNOL, LA JOLLA, CA 92037 USA

[5] TOHOKU UNIV, INST DEV AGING & CANC, DEPT MOL IMMUNOL, SENDAI, MIYAGI 980, JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1997年 / 41卷 / 07期

关键词：

C5a receptor (C5aR); lipopolysaccharide (LPS); cDNA cloning;

D O I：

10.1111/j.1348-0421.1997.tb01894.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Activation of the complement cascade plays an esssential role in the early stages of inflammation. C5a and its receptor are particularly active in anaphylaxis., To determine the pathological roles played by C5a and C5a receptor (C5aR) in rats, we cloned C5aR cDNA and analyzed distribution of its mRNA in various organs including lung from an LPS-stimulated rat, Furthermore, we generated a polyclonal antiserum which specifically recognizes rat C5aR, as confirmed by its specific interaction with cells transfected with rat C5aR cDNA.

引用

页码：575 / 580

页数：6

共 15 条

[1] DERIVATION OF 2 DISTINCT ANAPHYLATOXIN ACTIVITIES FROM THIRD AND FIFTH COMPONENTS OF HUMAN COMPLEMENT [J].