Lipoplexes of dicationic gemini surfactants with DNA: Structural features of DNA compaction and transfection efficiency

被引：2

作者：

Faizullin, D. A. ^{[1
]}

Zuev, Yu. F. ^{[1
]}

Zakharova, L. Ya. ^{[2
]}

Pokrovsky, A. G. ^{[3
]}

Korobeinikov, V. A. ^{[3
]}

Mukhametzyanov, T. A. ^{[4
]}

Konovalov, A. I. ^{[2
]}

机构：

[1] Russian Acad Sci, Kazan Sci Ctr, Kazan Inst Biochem & Biophys, Moscow 117901, Russia

[2] Russian Acad Sci, Kazan Res Ctr, Arbuzov Inst Organ & Phys Chem, Kazan 420088, Tatarstan, Russia

[3] Novosibirsk State Univ, Novosibirsk 630090, Russia

[4] Kazan Volga Reg Fed Univ, Kazan 420008, Tatarstan, Russia

来源：

DOKLADY BIOCHEMISTRY AND BIOPHYSICS | 2015年 / 465卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

GENE DELIVERY; CIRCULAR-DICHROISM;

D O I：

10.1134/S1607672915060204

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The internal structure of DNA lipoplexes with hydroxyethylated alkylammonium gemini surfactants (GS) with high transfection activity was studied by circular dichroism. It was shown that the efficiency of transfection of HEK293T cells with the pEGFP-N1 circular plasmid was different from zero only in the region of existence of chiral supramolecular DNA-GS complexes and reaches a maximum at concentrations at which the spontaneous aggregation of components is observed.

引用

页码：432 / 435

页数：4

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