Hydrophilic metal-organic frameworks integrated uricase for wearable detection of sweat uric acid

被引:53
作者
Xiao, Jingyu [1 ]
Luo, Yong [1 ]
Su, Lei [1 ]
Lu, Jiafa [2 ]
Han, Wei [2 ]
Xu, Tailin [1 ]
Zhang, Xueji [1 ,2 ]
机构
[1] Shenzhen Univ, Sch Biomed Engn, Guangdong Key Lab Biomed Measurements & Ultrasoun, Shenzhen 518060, Guangdong, Peoples R China
[2] Shenzhen Univ Clin Med Acad, Shenzhen Univ Gen Hosp, Dept Emergency Med, 1098 Xueyuan Ave, Shenzhen 518000, Peoples R China
基金
中国国家自然科学基金;
关键词
Wearable electrochemical sensor; Sweat analysis; Microfluidic chip; MAF-7; UA analysis; GLUCOSE; SENSOR;
D O I
10.1016/j.aca.2022.339843
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The chemistry of the metal-organic frameworks (MOFs) coating may affect the biological functionality of the encapsulated biomacromolecules in harsh environment. Enzymes encapsulated in hydrophilic MAF-7 can retain high activity in harsh environment. We conducted this study to prepare a non-invasive wearable uircase@MAF7-based electrochemical sensor that can achieve accurate and sensitive detection of UA levels in sweat by integrating a flexible microfluidic chip and wireless electronic readout device. The flexible microfluidic chip enabled an easy and effective collection of sweat samples. MAF-7 protected enzyme activity by encapsulating uricase. The uricase@MAF-7-based electrochemical sensor enabled the highly sensitive detection of UA in the concentration range of 2??M???70 ??M with a detection limit of as low as 0.34 ??M. Additionally, we evaluated the utility of the sensor for monitoring UA levels in real sweat samples by means of a high purine dietary challenge. This personalized wearable sweat sensing device has a potential to be used for monitoring disease-related metabolites in daily life.
引用
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页数:7
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