Ultrafine particles affect experimental thrombosis in an in vivo hamster model

被引:252
作者
Nemmar, A
Hoylaerts, MF
Hoet, PHM
Dinsdale, D
Smith, T
Xu, HY
Vermylen, J
Nemery, B
Nemery, B
机构
[1] Katholieke Univ Leuven, Lab Pneumol, Unit Lung Toxicol, B-3000 Louvain, Belgium
[2] Katholieke Univ Leuven, Ctr Mol & Vasc Biol, B-3000 Louvain, Belgium
[3] MRC, Toxicol Unit, Leicester, Leics, England
关键词
air pollution; cardiovascular effects; hemostasis; platelets; ultrafine particles;
D O I
10.1164/rccm.200110-026OC
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Particulate air pollution is associated with cardiovascular morbidity and mortality. To investigate this association, we studied the effect of ultrafine (60 nm) polystyrene particles on thrombus formation in a hamster model after intravenous and intratracheal administration of unmodified, car boxylate-polystyrene, or amine-polystyrene particles. Unmodified particles had no effect on thrombosis up to 5 mg/kg. Carboxylate-polystyrene particles significantly inhibited thrombus formation at 500 and 100 mug/kg body weight but not at 50 mug/kg body weight. In contrast, amine-polystyrene particles significantly enhanced thrombosis at 500 and 50 mug/kg body weight but not at 5 mug/kg body weight. Similarly, the intratracheal instillation of 5,000 jig of amine-polystyrene particles significantly increased thrombus formation. The unmodified particles and carboxylate-polystyrene particles had no effect. During platelet aggregation in human platelet-rich plasma, induced with 1.25 muM ADP, unmodified particles had no effect up to 100 mug/ml, and carboxylate-polystyrene particles weakly enhanced platelet aggregation at 25 to 100 mug/ml. However, amine-polystyrene particles (50 and 100 mug/ml) induced platelet aggregation themselves and strongly increased the ADP-induced aggregation. We conclude that the presence of (ultrafine) particles in the circulation may affect hemostasis. The observed in vivo prothrombotic tendency results, at least in part, from platelet activation by positively charged amine-polystyrene particles.
引用
收藏
页码:998 / 1004
页数:7
相关论文
共 44 条
[1]   Stimulation of human and rat alveolar macrophages by urban air particulates: Effects on oxidant radical generation and cytokine production [J].
Becker, S ;
Soukup, JM ;
Gilmour, MI ;
Devlin, RB .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1996, 141 (02) :637-648
[2]   AIRWAY HYPERRESPONSIVENESS IN GUINEA-PIGS EXPOSED TO ACID-COATED ULTRAFINE PARTICLES [J].
CHEN, LC ;
MILLER, PD ;
AMDUR, MO ;
GORDON, T .
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH, 1992, 35 (03) :165-174
[3]   Deposition of charged particles on lung airways [J].
Cohen, BS ;
Xiong, JQ ;
Fang, CP ;
Li, W .
HEALTH PHYSICS, 1998, 74 (05) :554-560
[4]   AN ASSOCIATION BETWEEN AIR-POLLUTION AND MORTALITY IN 6 UNITED-STATES CITIES [J].
DOCKERY, DW ;
POPE, CA ;
XU, XP ;
SPENGLER, JD ;
WARE, JH ;
FAY, ME ;
FERRIS, BG ;
SPEIZER, FE .
NEW ENGLAND JOURNAL OF MEDICINE, 1993, 329 (24) :1753-1759
[5]   AIR-POLLUTION AND DAILY MORTALITY - ASSOCIATIONS WITH PARTICULATES AND ACID AEROSOLS [J].
DOCKERY, DW ;
SCHWARTZ, J ;
SPENGLER, JD .
ENVIRONMENTAL RESEARCH, 1992, 59 (02) :362-373
[6]   Role of chondroitin 4-sulphate as a receptor for polycation induced human platelet aggregation [J].
Donato, JL ;
Marcondes, S ;
Antunes, E ;
Nogueira, MD ;
Nader, HB ;
Dietrich, CP ;
Rendu, F ;
deNucci, G .
BRITISH JOURNAL OF PHARMACOLOGY, 1996, 119 (07) :1447-1453
[7]   Soluble transition metals mediate residual oil fly ash induced acute lung injury [J].
Dreher, KL ;
Jaskot, RH ;
Lehmann, JR ;
Richards, JH ;
McGee, JK ;
Ghio, AJ ;
Costa, DL .
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH, 1997, 50 (03) :285-305
[8]   Oil fly ash-induced elevation of plasma fibrinogen levels in rats [J].
Gardner, SY ;
Lehmann, JR ;
Costa, DL .
TOXICOLOGICAL SCIENCES, 2000, 56 (01) :175-180
[9]   Adverse health effects of PM(10) particles: Involvement of iron in generation of hydroxyl radical [J].
Gilmour, PS ;
Brown, DM ;
Lindsay, TG ;
Beswick, PH ;
MacNee, W ;
Donaldson, K .
OCCUPATIONAL AND ENVIRONMENTAL MEDICINE, 1996, 53 (12) :817-822
[10]   Polyanions protect against the in vitro pulmonary toxicity of polycationic paint components associated with the Ardystil syndrome [J].
Hoet, PHM ;
Gilissen, L ;
Nemery, B .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2001, 175 (02) :184-190