Cryo-electron tomography: A tool for in situ structural analysis of macromolecular complexes

被引：3
作者：
Chung, Jeong Min [1 ]
Jung, Hyun Suk [1 ]
机构：
[1] Kangwon Natl Univ, Coll Nat Sci, Dept Biochem, 1 Kangwondaehak Gil, Chuncheon Si 24341, Gangwon Do, South Korea
来源：
APPLIED SPECTROSCOPY REVIEWS | 2018年 / 53卷 / 2-4期
基金：
新加坡国家研究基金会;
关键词：
Transmission electron microscopy; cryo-electron microscopy; cryo-electron tomography; direct electron detector; subtomogram averaging; ELECTRON-MICROSCOPY; MOLECULAR ARCHITECTURE; CRYO-EM; HIV-1; BIOLOGY; CELL; RECONSTRUCTION; PROTEASOMES; DETECTORS; PARTICLES;
D O I：
10.1080/05704928.2017.1328426
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
Conventional techniques for structural analysis have intrinsic limitations, including damage or distortion of the functional conformation of a protein complex during purification steps. Recently, a promising electron microscopy technique, cryo-electron tomography (cryo-ET), has been used to obtain insights regarding the molecular assemblies that drive cellular processes. This review focuses on the introduction of the cryo-ET and its recent improvement of resolution, in combination with the development of hardware and software such as direct electron detectors and sub-tomogram averaging techniques. Although cryo-ET still has several challenges, it can provide a glimpse into the interaction and function of macromolecules in their native environment.
引用
收藏
页码：195 / 202
页数：8
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