Volatile general anaesthetic actions on recombinant nACh(alpha 7), 5-HT3 and chimeric nACh(alpha 7)-5-HT3 receptors expressed in Xenopus oocytes

被引：63

作者：

Zhang, L

Oz, M

Stewart, RR

Peoples, RW

Weight, FF

机构：

[1] Lab. of Molec. and Cell. Neurbio., Natl. Inst. Alcohol Abuse Alcoholism, National Institutes of Health, Bethesda

[2] LMCN, NIAAA, NIH, Rockville, MD 20852

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 1997年 / 120卷 / 03期

关键词：

anaesthetics; receptors; ion channels; molecular chimera; acetylcholine receptor; 5-hydroxytryptamine receptor;

D O I：

10.1038/sj.bjp.0700934

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect of halothane and isoflurane was studied on the function of recombinant neurotransmitter receptors expressed in Xenopus oocytes. Both anaesthetics inhibited nicotinic acetylcholine type alpha 7 (nACh(alpha 7)) receptor-mediated responses, potentiated 5-hydroxytryptamine type 3 (5-HT3) receptor-mediated responses at low agonist concentrations, and inhibited the function of a chimeric receptor (with the N-terminal domain from the nACh(alpha 7) receptor and the transmembrane and C-terminal domains from the 5-HT3 receptor) in a manner similar to that of the nACh(alpha 7) receptor. Since the N-terminal domain of the chimeric receptor was from the nAC(alpha 7) receptor, the observations suggest that the inhibition involves the N-terminal domain of the receptor.

引用

页码：353 / 355

页数：3

共 10 条

[1] CHIMERIC NICOTINIC SEROTONERGIC RECEPTOR COMBINES DISTINCT LIGAND-BINDING AND CHANNEL SPECIFICITIES [J].

EISELE, JL ;

BERTRAND, S ;

GALZI, JL ;

DEVILLERSTHIERY, A ;

CHANGEUX, JP ;

BERTRAND, D .

NATURE, 1993, 366 (6454) :479-483

[2]

FORMAN SA, 1995, MOL PHARMACOL, V48, P574

[3] MOLECULAR AND CELLULAR MECHANISMS OF GENERAL-ANESTHESIA [J].

FRANKS, NP ;

LIEB, WR .

NATURE, 1994, 367 (6464) :607-614

[4] SELECTIVE ACTIONS OF VOLATILE GENERAL-ANESTHETICS AT MOLECULAR AND CELLULAR-LEVELS [J].

FRANKS, NP ;

LIEB, WR .

BRITISH JOURNAL OF ANAESTHESIA, 1993, 71 (01) :65-76

[5] Actions of general anaesthetics on 5-HT3 receptors in N1E-115 neuroblastoma cells [J].

Jenkins, A ;

Franks, NP ;

Lieb, WR .

BRITISH JOURNAL OF PHARMACOLOGY, 1996, 117 (07) :1507-1515

[6]

MACHU TK, 1994, J PHARMACOL EXP THER, V271, P898

[7] PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF THE 5HT3 RECEPTOR, A SEROTONIN-GATED ION CHANNEL [J].

MARICQ, AV ;

PETERSON, AS ;

BRAKE, AJ ;

MYERS, RM ;

JULIUS, D .

SCIENCE, 1991, 254 (5030) :432-437

[8] ACTIONS OF GENERAL-ANESTHETICS ON A NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR IN ISOLATED IDENTIFIED NEURONS OF LYMNAEA-STAGNALIS [J].

MCKENZIE, D ;

FRANKS, NP ;

LIEB, WR .

BRITISH JOURNAL OF PHARMACOLOGY, 1995, 115 (02) :275-282

[9] BRAIN ALPHA-BUNGAROTOXIN BINDING-PROTEIN CDNAS AND MABS REVEAL SUBTYPES OF THIS BRANCH OF THE LIGAND-GATED ION CHANNEL GENE SUPERFAMILY [J].

SCHOEPFER, R ;

CONROY, WG ;

WHITING, P ;

GORE, M ;

LINDSTROM, J .

NEURON, 1990, 5 (01) :35-48

[10] POTENTIATION OF 5-HT3 RECEPTOR-MEDIATED RESPONSES BY PROTEIN-KINASE-C ACTIVATION [J].

ZHANG, L ;

OZ, M ;

WEIGHT, FF .

NEUROREPORT, 1995, 6 (10) :1464-1468

← 1 →