A polymer-film inertial microfluidic sorter fabricated by jigsaw puzzle method for precise size-based cell separation

被引:48
作者
Zhu, Zhixian [1 ,2 ]
Wu, Dan [3 ,4 ]
Li, Shuang [5 ]
Han, Yu [1 ,2 ]
Xiang, Nan [1 ,2 ]
Wang, Cailian [3 ,5 ]
Ni, Zhonghua [1 ,2 ]
机构
[1] Southeast Univ, Sch Mech Engn, Nanjing 211189, Peoples R China
[2] Southeast Univ, Jiangsu Key Lab Design & Manufacture Micronano Bi, Nanjing 211189, Peoples R China
[3] Nanjing Med Univ, Nanjing 210011, Jiangsu, Peoples R China
[4] Jiangyin Peoples Hosp, Dept Oncol, Jiangyin 214400, Peoples R China
[5] Southeast Univ, Dept Oncol, Zhongda Hosp, Nanjing 210009, Peoples R China
基金
中国国家自然科学基金;
关键词
Inertial microfluidics; Spiral channel; Jigsaw puzzle method; Cell separation; Circulating tumor cells; CIRCULATING TUMOR-CELLS; CONTINUOUS PARTICLE SEPARATION; SPIRAL MICROCHANNELS; ULTRA-FAST; BLOOD; CANCER; TECHNOLOGIES;
D O I
10.1016/j.aca.2020.11.001
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A polymer-film inertial microfluidic jigsaw (PIMJ) sorter with trapezoidal spiral channels using the jigsaw puzzle method was proposed to realize precise and high-throughput rare cell separation. The PIMJ sorter was fabricated by assembling laser-patterned polymer-film layers of different thicknesses. After illustrating the conceptual design and fabrication process, the effects of the cross-sectional dimension, particle size, and operational flow rate on particle focusing were systematically explored under a broad flow rate range. Then, the separation performances of the PIMJ sorter were characterized using the binary particle mixture and the blood samples spiked with four types of tumor cells. The results indicated that the complete separation of the binary particles with a minimum size difference of 2 mu m was successfully realized at a high throughput up to 3000 mu L/min. A high recovery ratio of 90.57%-94.14% and a high purity of 48.67%-79.05% were achieved for the separation of rare tumor cells from white blood cells (WBCs). Finally, the PIMJ sorter was successfully employed for separating rare circulating tumor cells (CTCs) from the metastatic breast and lung cancer patients with a capture ratio of 7-226 CTCs per 5 mL sample. The results proved the high sensitivity and high reliability of the PIMJ sorter. The PIMJ sorter is expected to be a potential device for precise CTC separation towards the clinical applications. (C) 2020 Elsevier B.V. All rights reserved.
引用
收藏
页码:306 / 314
页数:9
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