Expression Analysis of the Stem Cell Marker Pw1/Peg3 Reveals a CD34 Negative Progenitor Population in the Hair Follicle

被引:8
作者
Besson, Vanessa [1 ]
Kyryachenko, Sergiy [1 ]
Janich, Peggy [2 ]
Benitah, Salvador A. [2 ,3 ,4 ]
Marazzi, Giovanna [1 ]
Sassoon, David [1 ]
机构
[1] Univ Pierre & Marie Curie Paris VI, Inst Cardiometabol & Nutr ICAN, UMRS 1166 INSERM, Stem Cells & Regenerat Med, Paris, France
[2] Barcelona Inst Sci & Technol, IRB Barcelona, Barcelona, Spain
[3] Catalan Inst Res & Adv Studies, ICREA, Barcelona, Spain
[4] ICREA, Barcelona, Spain
关键词
Pw1/Peg3; Bulge stem cells; Cell cycle; LABEL-RETAINING CELLS; DERMAL PAPILLA CELLS; MAMMALIAN EPIDERMIS; MOUSE EPIDERMIS; SKIN; NICHE; GROWTH; FATE; PROLIFERATION; HOMEOSTASIS;
D O I
10.1002/stem.2540
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Pw1/Peg3 is a parentally imprinted gene expressed in adult stem cells in every tissue thus far examined including the stem cells of the hair follicle. Using a Pw1/Peg3 reporter mouse, we carried out a detailed dissection of the stem cells in the bulge, which is a major stem cell compartment of the hair follicle in mammalian skin. We observed that PW1/Peg3 expression initiates upon placode formation during fetal development, coincident with the establishment of the bulge stem cells. In the adult, we observed that PW1/Peg3 expression is found in both CD34(+) and CD34(-) populations of bulge stem cells. We demonstrate that both populations can give rise to new hair follicles, reconstitute their niche, and self-renew. These results demonstrate that PW1/Peg3 is a reliable marker of the full population of follicle stem cells and reveal a novel CD34(-) bulge stem-cell population.
引用
收藏
页码:1015 / 1027
页数:13
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