Expression Analysis of the Stem Cell Marker Pw1/Peg3 Reveals a CD34 Negative Progenitor Population in the Hair Follicle

被引：9

作者：

Besson, Vanessa ^{[1
]}

Kyryachenko, Sergiy ^{[1
]}

Janich, Peggy ^{[2
]}

Benitah, Salvador A. ^{[2
,3
,4
]}

Marazzi, Giovanna ^{[1
]}

Sassoon, David ^{[1
]}

机构：

[1] Univ Pierre & Marie Curie Paris VI, Inst Cardiometabol & Nutr ICAN, UMRS 1166 INSERM, Stem Cells & Regenerat Med, Paris, France

[2] Barcelona Inst Sci & Technol, IRB Barcelona, Barcelona, Spain

[3] Catalan Inst Res & Adv Studies, ICREA, Barcelona, Spain

[4] ICREA, Barcelona, Spain

来源：

STEM CELLS | 2017年 / 35卷 / 04期

关键词：

Pw1/Peg3; Bulge stem cells; Cell cycle; LABEL-RETAINING CELLS; DERMAL PAPILLA CELLS; MAMMALIAN EPIDERMIS; MOUSE EPIDERMIS; SKIN; NICHE; GROWTH; FATE; PROLIFERATION; HOMEOSTASIS;

D O I：

10.1002/stem.2540

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Pw1/Peg3 is a parentally imprinted gene expressed in adult stem cells in every tissue thus far examined including the stem cells of the hair follicle. Using a Pw1/Peg3 reporter mouse, we carried out a detailed dissection of the stem cells in the bulge, which is a major stem cell compartment of the hair follicle in mammalian skin. We observed that PW1/Peg3 expression initiates upon placode formation during fetal development, coincident with the establishment of the bulge stem cells. In the adult, we observed that PW1/Peg3 expression is found in both CD34(+) and CD34(-) populations of bulge stem cells. We demonstrate that both populations can give rise to new hair follicles, reconstitute their niche, and self-renew. These results demonstrate that PW1/Peg3 is a reliable marker of the full population of follicle stem cells and reveal a novel CD34(-) bulge stem-cell population.

引用

页码：1015 / 1027

页数：13

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