Phthalate-induced oxidative stress and association with asthma-related airway inflammation in adolescents

被引：78

作者：

Franken, Carmen ^{[1
,2
]}

Lambrechts, Nathalie ^{[1
]}

Govarts, Eva ^{[1
]}

Koppen, Gudrun ^{[1
]}

Den Hond, Elly ^{[3
]}

Ooms, Daniella ^{[1
]}

Voorspoels, Stefan ^{[1
]}

Bruckers, Liesbeth ^{[4
]}

Loots, Ilse ^{[5
]}

Nelen, Vera ^{[3
]}

Sioen, Isabelle ^{[6
,7
]}

Nawrot, Tim S. ^{[8
,9
]}

Baeyens, Willy ^{[10
]}

Van Larebeke, Nicolas ^{[10
,11
]}

Schoeters, Greet ^{[1
,2
,12
]}

机构：

[1] Flemish Inst Technol Res VITO, Boeretang 200, B-2400 Mol, Belgium

[2] Univ Antwerp, Dept Biomed Sci, Antwerp, Belgium

[3] Prov Inst Hyg, Antwerp, Belgium

[4] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Hasselt, Belgium

[5] Univ Antwerp, Polit & Social Sci, Antwerp, Belgium

[6] Univ Ghent, Dept Publ Hlth, Ghent, Belgium

[7] Univ Ghent, Dept Food Safety & Food Qual, Ghent, Belgium

[8] Hasselt Univ, Ctr Environm Sci, Diepenbeek, Belgium

[9] Leuven Univ, Dept Publ Hlth & Primary Care, Leuven, Belgium

[10] Vrije Univ Brussel, Dept Analyt & Environm Chem, Brussels, Belgium

[11] Univ Ghent, Dept Radiotherapy & Nucl Med, Ghent, Belgium

[12] Univ Southern Denmark, Inst Publ Hlth, Dept Environm Med, Odense, Denmark

来源：

INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH | 2017年 / 220卷 / 02期

关键词：

Human biomonitoring; Oxidative stress; Phthalate; Doctor-diagnosed asthma; 8-hydroxydeoxyguanosine; POLYCYCLIC AROMATIC-HYDROCARBONS; PERSONAL CARE PRODUCTS; PRENATAL EXPOSURE; LUNG-CANCER; DI(2-ETHYLHEXYL)PHTHALATE DEHP; URINARY CONCENTRATIONS; PRESCHOOL-CHILDREN; NLRP3; INFLAMMASOME; ATOPIC-DERMATITIS; INJECTION MODEL;

D O I：

10.1016/j.ijheh.2017.01.006

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Background: In Belgium, around 8.5% of the children have asthmatic symptoms. Increased asthma risk in children has been reported in relation to exposure to phthalate plasticizers but the underlying mechanisms are largely unknown. Aim: The aim of this study was to identify if oxidative stress, assessed by excision of 8-hydroxydeoxyguanosine (8-OHdG) from damaged DNA, is an intermediate marker for the association between phthalate exposure and doctor-diagnosed asthma. Material and methods: In 41814-15-year-old youngsters, recruited as a representative sample of residents of Flanders (Belgium), personal exposure to phthalates was assessed by measuring phthalate metabolites in urine: mono(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono(2-ethyl-5-oxohexyl) phthalate (MEOHP), mono-n-butyl phthalate (MnBP), mono-benzyl phthalate (MBzP), mono-isobutyl phthalate (MiBP) and mono-ethyl phthalate (MEP). Analysis of 8-OHdG in urine was used as a sensitive biomarker of oxidative stress at the level of DNA. The presence of doctor diagnosed asthma was elicited by a self-administered questionnaire. Associations were assessed using multiple linear and logistic regression models. Mediation was tested using Baron and Kenny's regression approach. Results: A significant increased risk of a youngster being diagnosed with asthma was found for both urinary MnBP (metabolite of dibutyl phthalate (DBP)) and the sum of the three di(2-ethylhexyl) phthalate metabolites (Sigma DEHP = MEHP + MEHHP + MEOHP), with respective odds ratio of 1.84 [95% CI: 1.02,3.32] for MnBP and 1.94 [95% CI: 1.07, 3.51] for Sigma DEHP.In addition, we observed significant associations between all urinary phthalate metabolites and increased urinary levels of 8-OHdG. The associations were stronger in girls than in boys. We did not found evidence that 8-OHdG was associated with doctor-diagnosed asthma. Conclusion: The results of our study are in line with other findings from epidemiological surveys and raise further concern about DEHP and DBP as risk factors for asthma, however, the underlying mechanisms are not yet well understood. (C) 2017 Elsevier GmbH. All rights reserved.

引用

页码：468 / 477

页数：10

共 76 条

[1] NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases [J].