NON-CONTACT HIGH-FREQUENCY ULTRASOUND MICROBEAM STIMULATION FOR STUDYING MECHANOTRANSDUCTION IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS

被引：30

作者：

Hwang, Jae Youn ^{[1
]}

Lim, Hae Gyun ^{[2
]}

Yoon, Chi Woo ^{[2
]}

Lam, Kwok Ho ^{[3
]}

Yoon, Sangpil ^{[2
]}

Lee, Changyang ^{[2
]}

Chiu, Chi Tat ^{[2
]}

Kang, Bong Jin ^{[2
]}

Kim, Hyung Ham ^{[2
]}

Shung, K. Kirk ^{[2
]}

机构：

[1] Daegu Gyeongbuk Inst Sci & Technol, Dept Informat & Commun Engn, Taegu 711873, South Korea

[2] Univ So Calif, Dept Biomed Engn, Los Angeles, CA 90089 USA

[3] Hong Kong Polytech Univ, Dept Elect Engn, Kowloon, Hong Kong, Peoples R China

来源：

ULTRASOUND IN MEDICINE AND BIOLOGY | 2014年 / 40卷 / 09期

基金：

美国国家卫生研究院;

关键词：

High-frequency ultrasound microbeam; Mechanotransduction; Human umbilical vein endothelial cells; Calcium fluorescence imaging; FLUID SHEAR-STRESS; CHONDROCYTES; PROLIFERATION; HETEROGENEITY; TRANSDUCERS; MORPHOLOGY; INTENSITY; GRADIENTS; VIABILITY; MATRICES;

D O I：

10.1016/j.ultrasmedbio.2014.03.018

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechano-transduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. Avariety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified. (E-mail: jyhwang@dgist.ac.kr) (C) 2014 World Federation for Ultrasound in Medicine & Biology.

引用

页码：2172 / 2182

页数：11

共 35 条

[1] Mechanotransduction and endothelial cell homeostasis: the wisdom of the cell [J].