Improved method of analysis of biomass sugars using high-performance liquid chromatography

被引:37
作者
Agblevor, FA [1 ]
Murden, A
Hames, BR
机构
[1] Virginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USA
[2] Natl Renewable Energy Lab, Golden, CO 80401 USA
关键词
biomass hydrolysates; biomass sugars; glucose; HPLC analysis; xylose;
D O I
10.1023/B:BILE.0000036596.95796.42
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The precise quantitative analysis of biomass derived sugars is a very important step in the conversion of biomass feedstocks to fuels and chemicals. However, the most accurate method of biomass sugar analysis is based on the gas chromatography analysis of derivatized sugars either as alditol acetates or trimethylsilanes. The derivatization method is time-consuming but the alternative HPLC method cannot resolve most sugars found in biomass hydrolysates. We have demonstrated for the first time that by careful manipulation of the HPLC mobile phase, biomass monomeric sugars ( arabinose, xylose, fructose, glucose, mannose, and galactose) can be analyzed quantitatively and there is excellent baseline resolution of all the sugars. This was demonstrated for both standard sugars and corn stover hydrolysates. Our method can also be used to analyze dimmeric sugars (cellobiose and sucrose).
引用
收藏
页码:1207 / 1210
页数:4
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